Modulation of gene expression in CD4+ T lymphocytes following in vitro HIV infection: a comparison between human and chimpanzee

Autor: Pol-André Apoil, Antoine Blancher, Arnaud Gleizes, Lionel Forestier, Jacques Izopet, Christophe Pasquier, Peter Winterton, Raymond Julien, Bénédicte Puissant-Lubrano
Přispěvatelé: Laboratoire d'Immunogénétique Moléculaire (LIMT), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, CHU Toulouse [Toulouse], Université de Limoges (UNILIM), Université Fédérale Toulouse Midi-Pyrénées, Laboratoire de Virologie [Toulouse]
Rok vydání: 2015
Předmět:
Zdroj: VirusDisease
VirusDisease, Springer, 2015, 26 (1-2), pp.62-69. ⟨10.1007/s13337-015-0252-1⟩
ISSN: 2347-3517
2347-3584
Popis: International audience; Chimpanzees are susceptible to experimental infection by human deficiency virus (HIV)-1, but unlike humans, they exceptionally develop an immunodeficiency syndrome after HIV-1 inoculation. To explore the difference between human and chimpanzee, we analyzed the expression of 1547 genes of various functions in human or chimpanzee CD4+ lymphoblasts inoculated in vitro with HIV-1. We observed that, 1 day after HIV inoculation, fifty-eight genes were up-regulated in lymphoblasts of the three humans while their expression remained unchanged in lymphoblasts of the three chimpanzees. One gene is involved in adhesion of HIV (catenin-alpha), three in the immune response (semaphorin 4D, placental growth factor, IL-6), three in apoptosis (deleted in colorectal carcinoma, caspase 9 and FOXO1A). No difference between species was revealed for the expression of 373 genes related to glycosylation pathways. The in vitro human/chimpanzee comparison reveals new candidate genes up-regulated after inoculation with HIV-1 only in human lymphoblasts and which could be related to the higher sensitivity of human to HIV-induced AIDS.
Databáze: OpenAIRE
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