Membrane-integrated fermentation system for improving the optical purity of D-lactic acid produced during continuous fermentation
| Autor: | Sakayu Shimizu, Masahiro Henmi, Hideki Sawai, Kyungsu Na, Tetsu Yonehara, Katsushige Yamada, Shinichi Minegishi, Takashi Mimitsuka, Nanami Sasaki |
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| Rok vydání: | 2011 |
| Předmět: |
Batch fermentation
Optical Phenomena Applied Microbiology and Biotechnology Biochemistry Fermentation system Analytical Chemistry Continuous fermentation Bioreactors Organic chemistry D-Lactic Acid Lactic Acid Enantiomeric excess Molecular Biology Bacillales Chromatography L-Lactate Dehydrogenase Chemistry Organic Chemistry Cell Membrane food and beverages Stereoisomerism General Medicine Lactobacillus Membrane Fermentation Isomerization Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 75(12) |
| ISSN: | 1347-6947 |
| Popis: | This report describes the production of highly optically pure D-lactic acid by the continuous fermentation of Sporolactobacillus laevolacticus and S. inulinus, using a membrane-integrated fermentation (MFR) system. The optical purity of D-lactic acid produced by the continuous fermentation system was greater than that produced by batch fermentation; the maximum value for the optical purity of D-lactic acid reached 99.8% enantiomeric excess by continuous fermentation when S. leavolacticus was used. The volumetric productivity of the optically pure D-lactic acid was about 12 g/L/h, this being approximately 11-fold higher than that obtained by batch fermentation. An enzymatic analysis indicated that both S. laevolacticus and S. inulinus could convert L-lactic acid to D-lactic acid by isomerization after the late-log phase. These results provide evidence for an effective bio-process to produce D-lactic acid of greater optical purity than has conventionally been achieved to date. |
| Databáze: | OpenAIRE |
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