Alterations in mRNA expression of duodenal 1,25-dihydroxyvitamin D3 receptor and vitamin D-dependent calcium binding protein in aged wistar rats
| Autor: | Barnes J, DeLuca Hf, Liang Ct, Imanaka S |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Aging medicine.medical_specialty Brush border Duodenum chemistry.chemical_element Calcium Biology Biochemistry S100 Calcium Binding Protein G Endocrinology Dopamine receptor D3 Internal medicine Calcium-binding protein Gene expression Genetics medicine Animals RNA Messenger Rats Wistar Receptor Molecular Biology Messenger RNA Cell Biology Vitamin D-dependent calcium-binding protein Rats Intestinal Absorption chemistry Receptors Calcitriol |
| Zdroj: | Experimental Gerontology. 29:179-186 |
| ISSN: | 0531-5565 |
| DOI: | 10.1016/0531-5565(94)90049-3 |
| Popis: | Previously, we reported that uptake of calcium into isolated duodenal cells and duodenal brush border membrane vesicles decreased in senescence. Decreases in duodenal 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] receptor number and 9k vitamin D-dependent calcium binding protein (CaBP) were also observed in aged rats. In this study, we examined the steady state mRNA levels of duodenal 1,25-(OH)2D3 receptor and CaBP in both adult (6-month-old) and old (24-month-old) rats. We identified one major band of 4.4 kb for 1,25-(OH)2D3 receptor mRNA. The size of the transcript was not affected by age. The content of 1,25-(OH)2D3 receptor mRNA (normalized with poly(A)+ RNA) decreased 23% in the aged rat as compared to the adult rat. The expression of CaBP was also examined. A single band of 0.6 kb was observed for CaBP mRNA. The size of CaBP mRNA was not altered with age. However, the abundance of CaBP mRNA (normalized with poly(A)+ RNA) was reduced 20% in the senescent rat. Thus, the results in the present study were consistent with our previous findings that the number of 1,25-(OH)2D3 receptors and the level of CaBP declined in the aged rat. However, the precise mechanism leading to the age-related deficit in mRNA expression remains to be elucidated. |
| Databáze: | OpenAIRE |
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