Co-Expression of Chicken IL-2 and IL-7 Enhances the Immunogenicity and Protective Efficacy of a VP2-Expressing DNA Vaccine against IBDV in Chickens
| Autor: | Fei Zhong, Yuzhu Zuo, Jianlou Zhang, Jing-Hui Fan, Fengyang Wu, Shanshan Huo, Xing Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
IBDV VP2 DNA vaccine animal structures T cell viruses 030106 microbiology lcsh:QR1-502 Gene Expression Biology immunogenicity Antibodies Viral Infectious bursal disease virus chicken IL-2 Virus Article lcsh:Microbiology Infectious bursal disease DNA vaccination Cell Line 03 medical and health sciences Immunogenicity Vaccine Virology medicine Vaccines DNA Animals Humans Gene Poultry Diseases Viral Structural Proteins Immunity Cellular Immunogenicity Interleukin-7 Antibody titer medicine.disease Birnaviridae Infections Antibodies Neutralizing mutual enhancement chicken IL-7 030104 developmental biology Infectious Diseases medicine.anatomical_structure Host-Pathogen Interactions Interleukin-2 Gene vector Chickens |
| Zdroj: | Viruses, Vol 11, Iss 5, p 476 (2019) Viruses Volume 11 Issue 5 |
| ISSN: | 1999-4915 |
| Popis: | Chicken infectious bursal disease (IBD) is still incompletely controlled worldwide. Although IBD virus (IBDV) VP2 DNA vaccine was considered a safe vaccine for IBD prevention, the immunogenicity by itself remains poor, resulting in the failure of effectively protecting chickens from infection. We and others demonstrated that chicken IL-2 (chIL-2) and chIL-7 have the capacity to enhance the immunogenicity of the VP2 DNA vaccine. However, whether chIL-2 and chIL-7 can mutually enhance the immunogenicity of VP2 DNA vaccine and thereby augment the latter&rsquo s protection efficacy remains unknown. By using chIL-2/chIL-7 bicistronic gene vector to co-immunize the chickens together with the VP2 DNA vaccine, we now show that chIL-2 and chIL-7 significantly increased IBDV VP2-specific antibody titers, T cell proliferation, and IFN-&gamma production, resulting in the ultimate enhancement of vaccine-induced protection efficacy relative to that of chIL-2 or chIL-7 gene vectors alone. These results suggest that chIL-2 and chIL-7 can mutually enhance VP2 DNA vaccine&rsquo s efficacy, thereby establishing a concrete foundation for future optimization of IBDV VP2 DNA vaccine to prevent/treat chicken IBD. |
| Databáze: | OpenAIRE |
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