Specific VEGF sequestering and release using peptide-functionalized hydrogel microspheres
| Autor: | Michael W. Toepke, William L. Murphy, Sheeny K. Lan Levengood, Nicholas A. Impellitteri |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Serum
Vascular Endothelial Growth Factor A Materials science medicine.medical_treatment Molecular Sequence Data Biophysics Bioengineering Peptide Article Hydrogel Polyethylene Glycol Dimethacrylate Biomaterials Extracellular matrix chemistry.chemical_compound Downregulation and upregulation Gene expression medicine Human Umbilical Vein Endothelial Cells Animals Humans Amino Acid Sequence Microparticle Cell Proliferation chemistry.chemical_classification Growth factor Microspheres Cell biology Vascular endothelial growth factor chemistry Mechanics of Materials Ceramics and Composites Human umbilical vein endothelial cell Cattle Peptides Biomedical engineering Protein Binding |
| Popis: | Growth factor signaling plays an essential role in regulating processes such as tissue development, maintenance, and repair. Gene expression levels, diffusion, degradation, and sequestration by extracellular matrix components all play a role in regulating the concentration of growth factors within the cellular microenvironment. Herein, we describe the synthesis and characterization of hydrogel microspheres that mimic the ability of the native extracellular matrix to reversibly bind vascular endothelial growth factor (VEGF) out of solution. A peptide ligand derived from the VEGF receptor 2 (VEGFR2) was covalently incorporated into the hydrogel microspheres in order to achieve binding affinity and specificity. In addition to being able to both bind and release VEGF in a controllable manner, the microspheres were also shown to affect human umbilical vein endothelial cell (HUVEC) proliferation. The resulting microspheres may enable new strategies to specifically upregulate or downregulate growth factor signaling in the cellular microenvironment. |
| Databáze: | OpenAIRE |
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