Molecular dissection of the prototype foamy virus (PFV) RNA 5′-UTR identifies essential elements of a ribosomal shunt
| Autor: | Mikhail Schepetilnikov, Lyubov A. Ryabova, Gregory Schott, Mario Keller, Odon Thiébeauld, Konstantina Katsarou |
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| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Five prime untranslated region
Gene Products gag [SDV.BC]Life Sciences [q-bio]/Cellular Biology Ribosome Cell Line 03 medical and health sciences Open Reading Frames Eukaryotic translation Retrovirus Genetics Animals Spumavirus Peptide Chain Initiation Translational ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology 030302 biochemistry & molecular biology RNA biology.organism_classification Open reading frame RNA Viral Cauliflower mosaic virus 5' Untranslated Regions Ribosomes |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2009, 37, pp.5838-5847 |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The prototype foamy virus (PFV) is a nonpathogenic retrovirus that shows promise as a vector for gene transfer. The PFV (pre)genomic RNA starts with a long complex leader that can be folded into an elongated hairpin, suggesting an alternative strategy to cap-dependent linear scanning for translation initiation of the downstream GAG open reading frame (ORF). We found that the PFV leader carries several short ORFs (sORFs), with the three 5'-proximal sORFs located upstream of a structural element. Scanning-inhibitory hairpin insertion analysis suggested a ribosomal shunt mechanism, whereby ribosomes start scanning at the leader 5'-end and initiate at the downstream ORF via bypass of the central leader regions, which are inhibitory for scanning. We show that the efficiency of shunting depends strongly on the stability of the structural element located downstream of either sORFs A/A' or sORF B, and on the translation event at the corresponding 5'-proximal sORF. The PFV shunting strategy mirrors that of Cauliflower mosaic virus in plants; however, in mammals shunting can operate in the presence of a less stable structural element, although it is greatly improved by increasing the number of base pairings. At least one shunt configuration was found in primate FV (pre)genomic RNAs. |
| Databáze: | OpenAIRE |
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