A suprachiasmatic-independent circadian clock(s) in the habenula is affected by Per gene mutations and housing light conditions in mice
Autor: | Hélène Hamm, Jorge E. Mendoza, Nora L. Salaberry, Marie-Paule Felder-Schmittbuhl |
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Přispěvatelé: | Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
Rok vydání: | 2018 |
Předmět: |
endocrine system
Time Factors Histology [SDV]Life Sciences [q-bio] Photoperiod Period (gene) Circadian clock Biology 050105 experimental psychology Tissue Culture Techniques 03 medical and health sciences 0302 clinical medicine Reward Circadian Clocks Animals Epithalamus 0501 psychology and cognitive sciences Circadian rhythm Luciferases ComputingMilieux_MISCELLANEOUS Lighting Mice Knockout Habenula Motivation Behavior Animal Suprachiasmatic nucleus General Neuroscience 05 social sciences Period Circadian Proteins Housing Animal Cell biology Mice Inbred C57BL CLOCK PER2 Affect Mutation Suprachiasmatic Nucleus Anatomy Locomotion 030217 neurology & neurosurgery |
Zdroj: | Brain Structure and Function Brain Structure and Function, Springer Verlag, 2019, 224 (1), pp.19-31. ⟨10.1007/s00429-018-1756-4⟩ |
ISSN: | 1863-2661 1863-2653 |
Popis: | For many years, the suprachiasmatic nucleus (SCN) was considered as the unique circadian pacemaker in the mammalian brain. Currently, it is known that other brain areas are able to oscillate in a circadian manner. However, many of them are dependent on, or synchronized by, the SCN. The Habenula (Hb), localized in the epithalamus, is a key nucleus for the regulation of monoamine activity (dopamine, serotonin) and presents circadian features; nonetheless, the clock properties of the Hb are not fully described. Here, we report, first, circadian expression of clock genes in the lateral habenula (LHb) under constant darkness (DD) condition in wild-type mice which is disturbed in double Per1−/−-Per2Brdm1 clock-mutant mice. Second, using Per2::luciferase transgenic mice, we observed a self-sustained oscillatory ability (PER2::LUCIFERASE bioluminescence rhythmicity) in the rostral and caudal part of the Hb of arrhythmic SCN-ablated animals. Finally, in Per2::luciferase mice exposed to different lighting conditions (light-dark, constant darkness or constant light), the period or amplitude of PER2 oscillations, in both the rostral and caudal Hb, were similar. However, under DD condition or from SCN-lesioned mice, these two Hb regions were out of phase, suggesting an uncoupling of two putative Hb oscillators. Altogether, these results suggest that an autonomous clock in the rostral and caudal part of the Hb requires integrity of circadian genes to tick, and light information or SCN innervation to keep synchrony. The relevance of the Hb timing might reside in the regulation of circadian functions linked to motivational (reward) and emotional (mood) processes. |
Databáze: | OpenAIRE |
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