GSK3 activity is a cell fate switch that balances the ratio of vascular cell type
| Autor: | Masato Saito, Takayuki Tamaki, Yasuko Ozawa, Kiminori Toyooka, Makiko Naito, Satoyo Oya, Yuki Kondo, Tomoyuki Furuya, Hiroo Fukuda, Mayuko Sato, Mayumi Wakazaki, Ykä Helariutta |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences Cell type Chemistry fungi Complex formation Mutant food and beverages Cell fate determination Photosynthesis 01 natural sciences Cell biology 03 medical and health sciences Phloem Kinase activity Function (biology) 030304 developmental biology 010606 plant biology & botany |
| Popis: | The phloem transports photosynthetic assimilates and signalling molecules. It mainly consists of sieve elements (SEs), which act as “highways” for transport, and companion cells (CCs), which serve as “gates” to load/unload cargos. Though SEs and CCs function together, it remains unknown what determines the ratio of SE/CC in the phloem. In this study, we develop a novel culture system for CC differentiation named VISUAL-CC, which reconstitutes the SE-CC complex formation. Comparative expression analysis in VISUAL-CC reveals that SE and CC differentiation tends to show negative correlation, while total phloem differentiation is unchanged. This varying SE/CC ratio is largely dependent on GSK3 kinase activity. Indeed, gsk3 hextuple mutants possess much more SEs and less CCs in planta. Conversely, gsk3 gain-of-function mutants induced by phloem-specific promoter partially increased the CC ratio. Taken together, GSK3 activity appears to function as a cell fate switch in the phloem, thereby balancing the SE/CC ratio. |
| Databáze: | OpenAIRE |
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