Clinical and experimental study on angiopoietin-like protein 8 associated with proliferative diabetic retinopathy
| Autor: | Xiu-Rong Gong, Hong Wang, Chang-Xia Dong, Mei Dong, He-Ying Gao, Cai-Ping Song, Chun-Ping Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
proliferation retinal pigment epithelium angiopoietin-like protein 8 Western blot lcsh:Ophthalmology Internal medicine Medicine E2F2 biology medicine.diagnostic_test business.industry Cell growth Diabetic retinopathy medicine.disease Streptozotocin Proliferating cell nuclear antigen Ophthalmology Endocrinology Basic Research lcsh:RE1-994 biology.protein Hemoglobin business Cyclin A1 medicine.drug proliferative diabetic retinopathy |
| Zdroj: | International Journal of Ophthalmology, Vol 10, Iss 12, Pp 1819-1823 (2017) |
| Popis: | AIM: To confirm the role of angiopoietin-like protein 8 (Angptl 8) in proliferative diabetic retinopathy (PDR). METHODS: The sera and aqueous humor of 10 PDR patients and 10 non-diabetic retinopathy (NDR) patients (idiopathic macular hole patients) were collected and the expression of Angptl 8 was detected by enzyme linked immune-sorbent assay (ELISA). Experimental diabetes mice model was induced with streptozotocin. The expression of glycosylated hemoglobin and Angptl 8 in sera was detected. Recombinant Angptl 8 was re-infused into wild type (WT) diabetic mice and spatial frequency threshold and contrast sensitivity were measured. In vitro retinal pigment epithelium (RPE) were stimulated by recombinant Angptl 8 for 24h. MMT assay were used to detect cell proliferation. At the same time, qRT-PCR and Western blot was used to measure the expression of proliferation-related factors in PRE cells. RESULTS: The expression of Angptl 8 was markedly increased in the sera and aqueous humor of PDR patients (F=99.02, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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