A High-Throughput DNA-Sequencing Approach for Determining Sources of Fecal Bacteria in a Lake Superior Estuary
| Autor: | Chan Lan Chun, Christopher Staley, Michael J. Sadowsky, Ping Wang, B. J. Dalzell, Clairessa M. Brown |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030106 microbiology Zoology Biology DNA sequencing Feces 03 medical and health sciences fluids and secretions Environmental monitoring Animals Humans Environmental Chemistry Microbiome Effluent Human feces geography geography.geographical_feature_category Bacteria Ecology Estuary DNA Sequence Analysis DNA General Chemistry Fecal coliform Lakes Estuaries Water Microbiology Environmental Monitoring |
| Zdroj: | Environmental Science & Technology. 51:8263-8271 |
| ISSN: | 1520-5851 0013-936X |
| Popis: | Current microbial source-tracking (MST) methods, employed to determine sources of fecal contamination in waterways, use molecular markers targeting host-associated bacteria in animal or human feces. However, there is a lack of knowledge about fecal microbiome composition in several animals and imperfect marker specificity and sensitivity. To overcome these issues, a community-based MST method has been developed. Here, we describe a study done in the Lake Superior-Saint Louis River estuary using SourceTracker, a program that calculates the source contribution to an environment. High-throughput DNA sequencing of microbiota from a diverse collection of fecal samples obtained from 11 types of animals (wild, agricultural, and domesticated) and treated effluent (n = 233) was used to generate a fecal library to perform community-based MST. Analysis of 319 fecal and environmental samples revealed that the community compositions in water and fecal samples were significantly different, allowing for the determination of the presence of fecal inputs and identification of specific sources. SourceTracker results indicated that fecal bacterial inputs into the Lake Superior estuary were primarily attributed to wastewater effluent and, to a lesser extent, geese and gull wastes. These results suggest that a community-based MST method may be another useful tool for determining sources of aquatic fecal bacteria. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|