Adult-onset hyperthyroidism impairs spatial learning: possible involvement of mitogen-activated protein kinase signaling pathways
| Autor: | Burak Tan, S. Bitiktas, Başak Kandemir, Narin Liman, S. Kavraal, Hamiyet Donmez-Altuntas, Nurcan Dursun, Cem Süer, Işil Aksan-Kurnaz |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Male medicine.medical_specialty MAP Kinase Signaling System p38 mitogen-activated protein kinases Spatial Learning Morris water navigation task Water maze Hippocampus Hyperthyroidism p38 Mitogen-Activated Protein Kinases 03 medical and health sciences 0302 clinical medicine Western blot Internal medicine medicine Reaction Time Hippocampus (mythology) Animals RNA Messenger Rats Wistar Protein kinase A Maze Learning medicine.diagnostic_test business.industry Learning Disabilities General Neuroscience CREB-Binding Protein Rats Blot Disease Models Animal Thyroxine 030104 developmental biology Endocrinology Mental Recall business 030217 neurology & neurosurgery |
| Popis: | Given evidence that mitogen-activated protein kinase (MAPK) activation is part of the nongenomic actions of thyroid hormones, we investigated the possible consequences of hyperthyroidism for the cognitive functioning of adult rats. Young adult rats were treated with L-thyroxine or saline. Twenty rats in each group were exposed to Morris water maze testing, measuring their performance in a hidden-platform spatial task. In a separate set of rats not exposed to Morris water maze testing (untrained rats), the expression and phosphorylated levels of p38-MAPK and of its two downstream effectors, Elk-1 and cAMP response element-binding protein, were evaluated using quantitative reverse transcriptase-PCR and western blotting. Rats with hyperthyroidism showed delayed acquisition of learning compared with their wildtype counterparts, as shown by increased escape latencies and distance moved on the last two trials of daily training in the water maze. The hyperthyroid rats, however, showed no difference during probe trials. Western blot analyses of the hippocampus showed that hyperthyroidism increased phosphorylated p38-MAPK levels in untrained rats. Although our study is correlative in nature and does not exclude the contribution of other molecular targets, our findings suggest that the observed impairments in acquisition during actual learning in rats with hyperthyroidism may result from the increased phosphorylation of p38-MAPK. Copyright (C) 2016 Wolters Kluwer Health, Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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