PLOS ONE
| Autor: | James H. Fowler, Josephine Braun, Bruce A. Rideout, Carmel L. Witte, Laura L. Hungerford, Jennifer Burchell, Wayne Pfeiffer, Rebecca Papendick |
|---|---|
| Přispěvatelé: | Floto, Andres R, Population Health Sciences |
| Rok vydání: | 2020 |
| Předmět: |
Avian
Epidemiology INTRACELLULARE Single Nucleotide Polymorphisms Pathology and Laboratory Medicine Bird Genomics Mycobacterium avium avium Genotype Medicine and Health Sciences WATER 2.1 Biological and endogenous factors Aetiology education.field_of_study Multidisciplinary biology Tuberculosis Avian Bird Genetics Fungal genetics Eukaryota Genomics NONTUBERCULOUS MYCOBACTERIA Mycobacterium Avium Complex Bacterial Pathogens Actinobacteria Zoo Medical Microbiology Genetic Epidemiology Vertebrates Medicine GENAVENSE Pathogens Infection OUTBREAKS Social Network Analysis Biotechnology Research Article General Science & Technology Science Mycobacterium genavense Population ABSCESSUS Mycology Basic Behavioral and Social Science Microbiology Mycobacterium Vaccine Related Birds MOLECULAR EPIDEMIOLOGY Behavioral and Social Science Genetics Tuberculosis Animals Fungal Genetics education Microbial Pathogens Whole genome sequencing Mycobacterium Infections Molecular epidemiology Whole Genome Sequencing Bacteria Organisms Biology and Life Sciences Mycobacteria biology.organism_classification AVIUM SUBSP-AVIUM Evolutionary biology Animal Genomics Amniotes Nontuberculous mycobacteria Animals Zoo Zoology Animal Genetics Mycobacterium avium |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 6, p e0252152 (2021) PloS one, vol 16, iss 6 |
| ISSN: | 1932-6203 |
| Popis: | This study combined a social network analysis and whole-genome sequencing (WGS) to test for general patterns of contagious spread of a mycobacterial infection for which pathways of disease acquisition are not well understood. Our population included 275 cases diagnosed with avian mycobacteriosis that were nested in a source population of 16,430 birds at San Diego Zoo Wildlife Alliance facilities from 1992 through mid-2014. Mycobacteria species were determined using conventional methods and whole genome sequencing (WGS). Mycobacterium avium avium (MAA) and Mycobacterium genavense were the most common species of mycobacteria identified and were present in different proportions across bird taxa. A social network for the birds was constructed from the source population to identify directly and indirectly connected cases during time periods relevant to disease transmission. Associations between network connectivity and genetic similarity of mycobacteria (as determined by clusters of genotypes separated by few single nucleotide polymorphisms, or SNPs) were then evaluated in observed and randomly generated network permutations. Findings showed that some genotypes clustered along pathways of bird connectivity, while others were dispersed throughout the network. The proportion of directly connected birds having a similar mycobacterial genotype was 0.36 and significant (pM. genavense. Evaluations of SNP distributions also showed genotypes of MAA were more related in connected birds than expected by chance; however, no significant patterns of genetic relatedness were identified for M. genavense, although data were sparse. Integrating the WGS analysis of mycobacteria with a social network analysis of their host birds revealed significant genetic clustering along pathways of connectivity, namely for MAA. These findings are consistent with a contagious process occurring in some, but not all, case clusters. |
| Databáze: | OpenAIRE |
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