Distribution patterns of calcium-binding proteins in pancreatic tissue of non-diabetic as well as type 2 diabetic rats and in rat insulinoma beta-cells (INS-1)
| Autor: | Sabine Wolgast, Elmar Peschke, Eckhard Mühlbauer, Ivonne Bazwinsky-Wutschke |
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| Rok vydání: | 2010 |
| Předmět: |
endocrine system
medicine.medical_specialty Histology Calmodulin Islets of Langerhans Calcium-binding protein Internal medicine Insulin-Secreting Cells medicine Animals Rats Wistar education Molecular Biology Pancreas education.field_of_study geography geography.geographical_feature_category biology Chemistry Pancreatic islets Calcium-Binding Proteins Rat Insulinoma Cell Biology Islet Immunohistochemistry Rats Medical Laboratory Technology Endocrinology medicine.anatomical_structure Diabetes Mellitus Type 2 biology.protein Insulinoma Calreticulin SECRETAGOGIN |
| Zdroj: | Histochemistry and cell biology. 134(2) |
| ISSN: | 1432-119X |
| Popis: | The present study dealt with the localization of different calcium-binding proteins (CaBPs) in the pancreatic tissue of non-diabetic and diabetic rats and in rat insulinoma beta-cells (INS-1). Transcripts of CaBPs displayed different expression levels in rat pancreatic tissue and INS-1 cells. Immunohistochemistry demonstrated that three of these proteins, calmodulin, calreticulin and calbindin-D28k, were located predominantly in the pancreatic islets (in both alpha- and beta-cells) of rats, showing weaker labeling of exocrine tissue. Secretagogin was exclusively found within islets. All CaBPs were also immunohistochemically detected in INS-1 cells. Immunohistochemical analysis demonstrates differences in CaBP distributions when comparing the pancreatic tissues of diabetic Goto-Kakizaki rats and non-diabetic Wistar rats. Pancreatic tissue in type 2 diabetic Goto-Kakizaki rats showed significantly higher transcript levels of all CaBPs compared to those in Wistar rats. These results indicate that alterations of CaBPs in pancreatic islets are associated with metabolic disturbances related to type 2 diabetes. |
| Databáze: | OpenAIRE |
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