The protective effect of puerarin on angiotensin II‑induced aortic aneurysm formation by the inhibition of NADPH oxidase activation and oxidative stress‑triggered AP‑1 signaling pathways
| Autor: | Jianxing He, Youfei Qi, Shunwu Chang, Zhan-Xiang Xiao, Jie Yue |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Cancer Research Oxidase test Reactive oxygen species NADPH oxidase biology Articles 030204 cardiovascular system & hematology Pharmacology medicine.disease_cause Angiotensin II 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Oncology chemistry Puerarin Apoptosis 030220 oncology & carcinogenesis biology.protein medicine Signal transduction Oxidative stress |
| Zdroj: | Oncology Letters. |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2018.9021 |
| Popis: | Puerarin, an active ingredient of Pueraria lobata, has a range of pharmacological effects and excellent pharmacodynamic properties. In the present study, the effect of puerarin on angiotensin II-induced aortic aneurysm formation and the potential underlying molecular mechanisms were examined. The results revealed that puerarin significantly suppressed the viability, and induced the apoptosis, of aneurysm-inducing cells in a time- and dose-dependent manner. Furthermore, treatment with puerarin significantly suppressed the production of reactive oxygen species (ROS) and the expression of matrix metalloproteinase-2 (MMP-2) protein in aneurysm cells. Puerarin treatment significantly increased caspase-9 and −3 activity, induced the protein expression of phosphorylated (p)-Jun and inhibited the protein expression of activator protein 1 (AP-1) in aneurysm cells. It was also demonstrated that Puerarin significantly suppressed the reduced nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase activity in aneurysm cells. Therefore, it was demonstrated that puerarin on suppressed the cell growth of angiotensin II-induced aortic aneurysm formation by affecting the rate of apoptosis, the generation of ROS, MMP-2, AP-1 and p-Jun protein expression and NADPH oxidase. |
| Databáze: | OpenAIRE |
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