Research on the ability of propionic acid and vitamin B12 biosynthesis by Propionibacterium freudenreichii strain T82
Autor: | E. Hac-Szymanczuk, E. Lipinska, Anna Bzducha-Wróbel, Alicja Synowiec, Kamil Piwowarek |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
0301 basic medicine Sucrose Propionibacterium Metabolite Acetates Acetic acid 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Food science Molecular Biology chemistry.chemical_classification Original Paper biology Vitamin B12 Carbon sources Propionibacterium freudenreichii Fructose General Medicine biology.organism_classification Propionic acid Vitamin B 12 030104 developmental biology chemistry Biochemistry DoE Propionate Propionates Bacteria |
Zdroj: | Antonie Van Leeuwenhoek |
ISSN: | 1572-9699 0003-6072 |
Popis: | The purpose of this study was to determine the potential for biosynthesis of propionic acid and vitamin B12 by Propionibacterium freudenreichii T82 in a medium containing various sources of carbon (glucose, fructose, and saccharose). These sugars are present in apple pomaces, which are the waste from the production of apple juice. Using statistical analysis design of experiments (DoE), the results allowed us to determine which sugars (carbon sources) exert the most beneficial influence on the biosynthesis of propionic acid and cobalamin. The highest production of propionic acid by the tested bacterial strain was obtained in a medium in which glucose accounted for at least 50% of the available carbon sources. Depending on the culture medium, the concentration of this metabolite ranged from 23 to 40 g/L. P. freudenreichii T82 produced the smallest amount of acid in medium in which the dominant nutrient source was saccharose. The results obtained indicated an inverse relationship between the amount of acid produced by the bacteria and vitamin B12 biosynthesis. Because of the high efficiency of propionic acid biosynthesis by P. freudenreichii T82, the prospect of using this strain to obtain propionate with the simultaneous disposal of waste materials (such as apple pomaces) which contain glucose and/or fructose is very promising. |
Databáze: | OpenAIRE |
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