Zn(ii), Cd(ii) and Hg(ii) saccharinate complexes with 2,6-bis(2-benzimidazolyl)pyridine as promising anticancer agents in breast and lung cancer cell lines via ROS-induced apoptosis
| Autor: | Ceyda Icsel, Seyma Aydinlik, Veysel T. Yilmaz, Muhittin Aygün |
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| Přispěvatelé: | Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü., İçsel, Ceyda, Yılmaz, Veysel T., L-7238-2018, AAI-3342-2021 |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Models
Molecular Lung Neoplasms Pyridines Pyridine Cytotoxic activities Palladium(II) Cell Apoptosis Antiproliferative activity Spectroscopic method MCF-7 cell line Spectroscopic analysis Crystallography X-Ray 01 natural sciences Vitro DNA-binding Synthesis Chemical structure Coordination Complexes Annexin 2 6-bis(benzimidazol-2-yl)pyridine Pathology Cytotoxic T cell Pyridine derivative Cells Cultured chemistry.chemical_classification Gel electrophoresis Lung cancer cells Crystal-structures Ligands synthesis Structure activity relation Molecular Structure 2 6 bis(2 benzimidazolyl)pyridine Chemistry inorganic & nuclear Chemistry Zinc medicine.anatomical_structure Antineoplastic agent Drug screening A-549 cell line MCF-7 Cells Derivatives Coordination compound Human Cadmium Cell death Electrophoresis Cell Survival Anti-cancer agents Breast tumor Antineoplastic Agents Breast Neoplasms 010402 general chemistry Molecular model Inorganic Chemistry Structure-Activity Relationship Dose response medicine Apoptotic cell death Humans Zinc compounds Benzimidazole derivative Lung tumor Cell Proliferation A549 cell Reactive oxygen species Cytotoxic activity Dose-Response Relationship Drug Inhibitors 010405 organic chemistry Cadmium compounds Inhibitory activity DNA X ray crystallography Mercury Complex Palladium 2-Phenylpyridine Biological organs Molecular biology 0104 chemical sciences Antibacterial Drug effect Metabolism chemistry A549 Cells Cell culture Mercury compounds Benzimidazoles Drug Screening Assays Antitumor Reactive Oxygen Species Reactive oxygen metabolite |
| Popis: | New Zn(ii), Cd(ii) and Hg(ii) complexes of saccharinate (sac) and 2,6-bis(2-benzimidazolyl)pyridine (bzimpy), [Zn(bzimpy)(2)](sac)(2)center dot 2H(2)O (Zn), [Cd(sac)(2)(bzimpy)] (Cd) and [Hg(sac)(2)(bzimpy)] (Hg), were prepared and fully characterized by spectroscopic methods and X-ray crystallography.In vitroanticancer screening in A549 (lung), MCF-7 (breast) and HT29 (colon) cell lines showed thatZnwas highly cytotoxic against A549 and MCF-7 cells with IC(50)values of 1.74 +/- 0.06 and 3.15 +/- 0.10 mu M, respectively, andHgdemonstrated potent cytotoxic activity in MCF-7 cells (8.61 +/- 0.98 mu M), whileCdand bzimpy exhibited moderate growth inhibitory activities in all of the cell lines. In addition, they showed significantly lower toxicity towards normal human breast epithelial MCF10A cells. Moreover, the complexes exhibited significantly high nuclease activity towards plasmid DNA and their interactions with DNA were assessed by gel electrophoresis and DNA docking.ZnandHginduced G0/G1 cell arrest and apoptotic cell death detectedviatypical DNA condensation/fragmentation, annexin V staining and caspase 3/7 activity in A549 and MCF-7 cells. These complexes further caused depolarization of mitochondria and oxidative damage of genomic DNA following excessive production of reactive oxygen species (ROS). Anadolu Üniversitesi |
| Databáze: | OpenAIRE |
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