Corticotropin-releasing factor receptor types 1 and 2 are differentially expressed in pre- and post-synaptic elements in the post-natal developing rat cerebellum
| Autor: | F. Shi, E. H. Blaauw, J. IJkema-Paassen, J. J. L. van der Want, Dharamdajal Kalicharan, Albert Gramsbergen, Jerome D. Swinny |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
endocrine system
Dendritic spine Blotting Western Purkinje cell Presynaptic Terminals Dendrite Neurotransmission Biology MOUSE Receptors Corticotropin-Releasing Hormone dendrite neuromodulator PROTEIN-COUPLED RECEPTORS Cerebellum medicine Animals Protein Isoforms PEPTIDE BRAIN Receptor Long-term depression parallel fibre PURKINJE-CELLS FACTOR CRF General Neuroscience CENTRAL-NERVOUS-SYSTEM Glutamate receptor LONG-TERM DEPRESSION Rats Inbred Strains Rats Microscopy Electron medicine.anatomical_structure Animals Newborn Synapses Synaptic plasticity ANTERIOR-PITUITARY CELLS GLUTAMATE RECEPTORS Neuroscience hormones hormone substitutes and hormone antagonists climbing fibre |
| Zdroj: | European Journal of Neuroscience, 18(3), 549-562. Blackwell Publishing |
| ISSN: | 0953-816X |
| Popis: | Corticotropin-releasing factor (CRF)-like proteins act via two G-protein-coupled receptors (CRF-R1 and CRF-R2) playing important neuromodulatory roles in stress responses and synaptic plasticity. The cerebellar expression of corticotropin-releasing factor-like ligands has been well documented, but their receptor localization has not. This is the first combination of a light microscopic and ultrastructural study to localize corticotropin-releasing factor receptors immunohistologically in the developing rat cerebellum. Both CRF-R1 and CRF-R2 were expressed in climbing fibres from early stages (post-natal day 3) to the adult, but CRF-R2 immmunoreactivity was only prominent throughout the molecular layer in the posterior cerebellar lobules. CRF-R1 immunoreactivity was concentrated in apical regions of Purkinje cell somata and later in primary dendrites exhibiting a diffuse cytoplasmic appearance. In Purkinje cells, CRF-R1 immunoreactivity was never membrane bound post-synaptically in dendritic spines while CRF-R2 immunoreactivity was found on plasmic membranes of Purkinje cells from post-natal day 15 onwards. We conclude that the localization of these receptors in cerebellar afferents implies their pre-synaptic control of the release of corticotropin-releasing factor-like ligands, impacting on the sensory information being transmitted from afferents. Furthermore, the fact that CRF-R2 is membrane bound at synapses, while CRF-R1 is not, suggests that ligands couple to CRF-R2 via synaptic transmission and to CRF-R1 via volume transmission. Finally, the distinct expression profiles of receptors along structural domains of Purkinje cells suggest that the role for these receptors is to modulate afferent inputs. |
| Databáze: | OpenAIRE |
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