Isolation of 45 exon-like fragments from 8p22→p21.3, a region that is commonly deleted in hepatocellular, colorectal, and non-small cell lung carcinomas
| Autor: | H Ohata, Y Fujiwara, K Izawa, M Isomura, Yusuke Nakamura, T Iwamasa, K. Chinen |
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| Rok vydání: | 1996 |
| Předmět: |
Yeast artificial chromosome
Carcinoma Hepatocellular Lung Neoplasms Tumor suppressor gene Molecular Sequence Data Biology Loss of heterozygosity Exon Gene mapping Carcinoma Non-Small-Cell Lung Genetics Humans Genes Tumor Suppressor Amino Acid Sequence Chromosomes Artificial Yeast Molecular Biology Genetics (clinical) Southern blot Contig Liver Neoplasms Chromosome Mapping Exons Cosmids Blotting Southern Cosmid Cancer research Chromosome Deletion Colorectal Neoplasms Chromosomes Human Pair 8 |
| Zdroj: | Cytogenetic and Genome Research. 75:190-196 |
| ISSN: | 1424-859X 1424-8581 |
| DOI: | 10.1159/000134480 |
| Popis: | The loss of heterozygosity that is frequently observed at chromosome 8p22→p21.3 in hepatocellular carcinomas (HCC), colorectal cancers (CRC), prostate cancers (PRC), and non-small cell lung cancers (NSCLC), suggests the presence there of one or more tumor suppressor genes associated with development and/or progression of these types of carcinomas. We have constructed a cosmid contig map of the target region by means of Southern hybridization and have isolated 45 exon-like fragments by exon amplification, using cosmid clones derived from three yeast artificial chromosomes (YACs). The map and the exon-like fragments reported here will be useful resources for isolation of the putative tumor suppressor gene(s) as well as other novel genes located in the 8p22→p21.3 region. |
| Databáze: | OpenAIRE |
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