A genome wide dosage suppressor network reveals genomic robustness
| Autor: | Jesse P. Frumkin, Arend Hintze, Eric M. Phizicky, Bruz Marzolf, Alpan Raval, Biranchi N. Patra, Nitin S. Baliga, Amardeep Kaur, Elizabeth J. Grayhack, Ashish Bhan, Jenna K. Tamashiro, David J. Galas, Anthony W. Sevold, Christoph Adami, Ravishankar R. Vallabhajosyula, Gitanjali Yadav, Bjørn Østman, Jory Schossau, Yoshiko Kon, Animesh Ray |
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| Přispěvatelé: | Ray, Animesh [0000-0002-0120-5820], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
cell division
0301 basic medicine Gene regulatory network Gene Dosage gene regulatory network genetic analysis Lethal Genome 0302 clinical medicine Gene Expression Regulation Fungal genetic parameters genome wide dosage suppressor genetics Gene Regulatory Networks Genetics Regulation of gene expression biology correlational study Biochemistry and Molecular Biology reproductive fitness gene expression regulation Genomics 3. Good health Fungal RNA polymerase fungal genome RNA polymerase II RNA Polymerase II Genome Fungal cell cycle regulation Cell Division Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae gene repression gene dosage DNA replication Biology Gene dosage Article 03 medical and health sciences Saccharomyces cerevisiae protein lethal gene gene expression profiling mutant protein interaction RNA synthesis Gene gene interaction Gene Expression Profiling Robustness (evolution) Computational Biology bacterial strain biology.organism_classification Gene expression profiling gene function 030104 developmental biology RNA processing Genes protein analysis Mutation gene expression Genes Lethal microarray analysis Genetic Fitness mutation metabolism genomic robustness Biokemi och molekylärbiologi 030217 neurology & neurosurgery |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Genomic robustness is the extent to which an organism has evolved to withstand the effects of deleterious mutations. We explored the extent of genomic robustness in budding yeast by genome wide dosage suppressor analysis of 53 conditional lethal mutations in cell division cycle and RNA synthesis related genes, revealing 660 suppressor interactions of which 642 are novel. This collection has several distinctive features, including high cooccurrence of mutant-suppressor pairs within protein modules, highly correlated functions between the pairs and higher diversity of functions among the co-suppressors than previously observed. Dosage suppression of essential genes encoding RNA polymerase subunits and chromosome cohesion complex suggests a surprising degree of functional plasticity of macromolecular complexes, and the existence of numerous degenerate pathways for circumventing the effects of potentially lethal mutations. These results imply that organisms and cancer are likely able to exploit the genomic robustness properties, due the persistence of cryptic gene and pathway functions, to generate variation and adapt to selective pressures. © 2016 The Author(s). |
| Databáze: | OpenAIRE |
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