Human Finger-Prick Induced Pluripotent Stem Cells Facilitate the Development of Stem Cell Banking
| Autor: | Tze Kai Tan, Hong Keetan Tan, Hu Li, Bing Lim, Chee Wai Wong, Yuin-Han Loh, Stuart A. Cook, Yoon Pin Lim, Dongrui Ma, Binxia Yang, Jun Lu, Christopher Kiu Choong Syn, Tong Ming Liu, Eng Lee Tan, Cheng Xu Delon Toh |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Somatic cell Induced Pluripotent Stem Cells food and beverages Cell Biology General Medicine Computational biology Biology Regenerative Medicine Regenerative medicine Peripheral blood Specimen Handling Fingers Immunology Humans Female Sample collection Stem cell Induced pluripotent stem cell Reprogramming Finger prick Biological Specimen Banks Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells Developmental Biology |
| Zdroj: | Stem Cells Translational Medicine. 3:586-598 |
| ISSN: | 2157-6580 2157-6564 |
| Popis: | Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients can be a good model for studying human diseases and for future therapeutic regenerative medicine. Current initiatives to establish human iPSC (hiPSC) banking face challenges in recruiting large numbers of donors with diverse diseased, genetic, and phenotypic representations. In this study, we describe the efficient derivation of transgene-free hiPSCs from human finger-prick blood. Finger-prick sample collection can be performed on a “do-it-yourself” basis by donors and sent to the hiPSC facility for reprogramming. We show that single-drop volumes of finger-prick samples are sufficient for performing cellular reprogramming, DNA sequencing, and blood serotyping in parallel. Our novel strategy has the potential to facilitate the development of large-scale hiPSC banking worldwide. |
| Databáze: | OpenAIRE |
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