Lack of association between high-risk human papillomaviruses and oral squamous cell carcinoma in young Japanese patients
| Autor: | Hiroyuki Harada, Kei-ichi Morita, Pradit Rushatamukayanunt, Hiroaki Shimamoto, Sho Matsukawa, Ken Omura, Hirofumi Tomioka |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Oncology
Adult Male Cancer Research medicine.medical_specialty Alcohol Drinking Epidemiology Biology law.invention Human Papillomavirus DNA Tests Japan law P16 ink4a Internal medicine medicine Biomarkers Tumor Humans Basal cell Papillomaviridae Polymerase chain reaction Cyclin-Dependent Kinase Inhibitor p16 Surrogate endpoint Papillomavirus Infections Public Health Environmental and Occupational Health HPV infection Age Factors medicine.disease Staining Immunology DNA Viral Etiology Carcinoma Squamous Cell Immunohistochemistry Female Mouth Neoplasms |
| Zdroj: | Asian Pacific journal of cancer prevention : APJCP. 15(10) |
| ISSN: | 2476-762X |
| Popis: | Background: Human papillomaviruses (HPV) may play an important role as one of the possible etiologies of oral squamous cell carcinoma (OSCC). The present study aimed to investigate the association between HPV and OSCC in young Japanese patients by examining the presence of HPV DNA and surrogate markers in OSCC tissues. Materials and Methods: Forty young patients with OSCC whose surgical specimens were available were analyzed and compared with 40 patients randomly recruited from a pool of patients aged >40 years. HPV DNA was detected using the polymerase chain reaction-based AMPLICOR ® HPV test, and surrogate markers of HPV infection were analyzed using immunohistochemical techniques to detect p16 INK4a and p53. Results: Only two (5%) young patients and one (2.5%) older patient were positive for HPV DNA. p16 INK4a overexpression was identified in six (15%) young patients. p53 staining levels were not high in tissues of most young patients (27 patients, 67.5%). HPV DNA status did not significantly correlate with p16 INK4a expression levels. Profiles of increased levels of p16 INK4a expression with diminished levels of p53 staining were not associated with the presence of HPV DNA. The combined p53 with p16 INK4a profiles were significantly correlated with alcohol consumption in younger patients (p=0.006). Conclusions: Results of the present study indicate that HPV is less likely to cause OSCC in young Japanese patients, and the p16 INK4a expression level is not an appropriate surrogate marker for |
| Databáze: | OpenAIRE |
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