Interaction between Bacillus subtilis YsxC and ribosomes (or rRNAs)
Autor: | Catherine Wicker-Planquart, Jean-Michel Jault |
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Přispěvatelé: | Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), ANR-08-BLAN-0143,P-loop proteins,Rôle et mécanisme de fonctionnement de nouvelles enzymes bactériennes(2008), Thomas, Frank, Blanc - Rôle et mécanisme de fonctionnement de nouvelles enzymes bactériennes - - P-loop proteins2008 - ANR-08-BLAN-0143 - BLANC - VALID, Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) |
Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Thermal shift assay
Protein Denaturation GTP' [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Protein subunit Blotting Western Biophysics Ribosome Subunits Small Bacterial Bacillus subtilis GTPase Ribosome Subunits Large Bacterial Biology Sodium Chloride Biochemistry Ribosome Guanosine Diphosphate GTP Phosphohydrolases Potassium Chloride 03 medical and health sciences Bacterial Proteins Structural Biology RNA Ribosomal 16S Enzyme Stability Genetics Molecular Biology 030304 developmental biology 50S 0303 health sciences Dose-Response Relationship Drug [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] 030306 microbiology Temperature Cell Biology Ribosomal RNA biology.organism_classification RNA Ribosomal 23S YsxC Ribosome binding Guanosine Triphosphate Ribosomes Protein Binding |
Zdroj: | FEBS Letters FEBS Letters, Wiley, 2015, 589 (9), pp.1026-32 FEBS Letters, 2015, 589 (9), pp.1026-32 |
ISSN: | 0014-5793 1873-3468 |
Popis: | International audience; YsxC is an essential P-loop GTPase, that binds to the 50S ribosomal subunit, and is required for the proper assembly of the ribosome. The aim of this study was to characterize YsxC ribosome interactions. The stoichiometry of YsxC ribosome subunit complex was evaluated. We showed that YsxC binding to the 50S ribosomal subunit is not affected by GTP, but in the presence of GDP the stoichiometry of YsxC-ribosome is decreased. YsxC GTPase activity was stimulated upon 50S ribosomal subunit binding. In addition, it is shown for the first time that YsxC binds both 16S and 23S ribosomal RNAs. |
Databáze: | OpenAIRE |
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