Effects of contrast media on calcium transients and motion in cultured ventricular cells
| Autor: | James A. Nelson, William H. Barry, Osami Kohmoto, G. A. Peeters, Wayne L. Davis, Don A. Bell |
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| Rok vydání: | 1988 |
| Předmět: |
Inotrope
medicine.medical_specialty Sucrose animal structures media_common.quotation_subject Heart Ventricles Iohexol chemistry.chemical_element Contrast Media Chick Embryo Calcium Diatrizoate Contractility Contraction amplitude Internal medicine medicine Contrast (vision) Animals Radiology Nuclear Medicine and imaging Cells Cultured media_common Diatrizoate Meglumine Chemistry Osmolar Concentration General Medicine Myocardial Contraction Drug Combinations Endocrinology Tonicity Calcium Channels medicine.drug |
| Zdroj: | Investigative radiology. 23(11) |
| ISSN: | 0020-9996 |
| Popis: | To investigate the mechanisms of the negative inotropic effects of contrast media, we superfused spontaneously contracting cultured chick embryo ventricular cells with Renografin-76 and iohexol (12% solutions), and hypertonic sucrose during simultaneous measurement of [Ca2+]i transients (indo-1) and motion (video-motion detector system). Exposure to contrast agents caused a significant reduction of contractility, with Renografin-76 having a much greater effect on amplitude of motion than iohexol. Renografin-76 significantly depressed [Ca2+]i transient amplitude, whereas iohexol had no effect. Addition of Ca2+ to correct for calcium binding by Renografin-76 completely reversed its depression of [Ca2+]i transients but only partially reversed the negative inotropic effects. Hypertonic sucrose caused a significant decrease in contraction amplitude, with no significant effects on [Ca2+]i transient amplitude. We conclude that the marked negative inotropic effect of Renografin-76 is caused by both calcium binding and hypertonicity. The less marked depression of contractility produced by iohexol likely is a result of hypertonicity and not caused by alteration of [Ca2+]i. |
| Databáze: | OpenAIRE |
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