Aspergillus carbonarius polygalacturonases purified by integrated membrane process and affinity precipitation for apple juice production
| Autor: | Sukumaran Umesh-Kumar, Ekambaram Nakkeeran, Rangaswamy Subramanian |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Environmental Engineering
medicine.medical_treatment Ultrafiltration Fractional Precipitation Bioengineering Chromatography Affinity Beverages medicine Amylase Pectinase Waste Management and Disposal chemistry.chemical_classification Chromatography Protease biology Renewable Energy Sustainability and the Environment Aspergillus niger Membranes Artificial General Medicine biology.organism_classification Systems Integration Aspergillus Polygalacturonase Enzyme Biochemistry chemistry DNA glycosylase Fruit Malus biology.protein Fermentation Specific activity |
| Zdroj: | Bioresource Technology. 102:3293-3297 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2010.10.048 |
| Popis: | Aspergillus carbonarius, when grown by submerged and solid-state fermentation, produces different molecular forms of polygalacturonase (PG; EC 3.2.1.15), among them a 42 kDa PG with a high specific activity of 7000 U/mg protein. When the enzymes were purified by integrated membrane process (IMP) and alginate affinity precipitation (AAP), the two processes concentrated different forms of the enzyme. The AAP process selectively purified and concentrated the high active PG whereas the IMP yielded different PGs and also amylase and protease. Evaluation of the AAP enzyme preparations for apple juice preparation under conditions usually employed commercially demonstrated that the high activity PG did not result in good juice clarity. With IMP processed enzymes, juice yields and clarity were similar to that obtained with commercial PG from A. niger. |
| Databáze: | OpenAIRE |
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