Effect of recombinant trichosanthin on proliferation of human cevical cancer Caski cells
| Autor: | Yanzhen Wu, Weihong Cao, Chengcheng You, Liming Huang, Ping-ping Peng, Hanxing Tan, Yan-lin Wang, Huamei Song |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Trichosanthin
Uterine Cervical Neoplasms Transfection law.invention law Cell Line Tumor medicine Humans Pharmacology (medical) MTT assay General Pharmacology Toxicology and Pharmaceutics Cell Proliferation Chemistry Cell growth Cell Cycle Cancer Cell cycle medicine.disease Molecular biology Recombinant Proteins Complementary and alternative medicine Cell culture Recombinant DNA Female |
| Zdroj: | China Journal of Chinese Materia Medica. |
| ISSN: | 1001-5302 |
| DOI: | 10.4268/cjcmm20111819 |
| Popis: | OBJECTIVE: To observe the effects of high expression of recombinant trichosanthin (rTCS) on the cell proliferation and cell cycle of human cervical cancer Caski cells. METHOD: Eukaryotic expression plasmid pcDNA3.1(-)/6His-TCS was constracted and stably transfected into Caski cells. RT-PCR,Western-blot were used to select the clones with rTCS high-expressing. Using pcDNA3.1(-)-transfected cells as the control, MTT assay and flowcytometry were used to elucidate the effects of rTCS high expression on cell growth and cycle regulation in Caski cells. RESULT: The Caski cells with stable high expression of rTCS was successfully established, which could inhibit the cell growth (P |
| Databáze: | OpenAIRE |
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