siRNA off-target effects in genome-wide screens identify signaling pathway members
| Autor: | Julja Burchard, Aly A. Khan, Eugen Buehler, Shane Marine, Marc Ferrer, Amit Bahl, Misha Rajaram |
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| Rok vydání: | 2012 |
| Předmět: |
Small interfering RNA
Genomics Biology Genome Article RNA interference Animals Humans RNA Small Interfering 3' Untranslated Regions Wnt Signaling Pathway Gene Gene Library Genetics Multidisciplinary Base Sequence Models Genetic Gene Expression Profiling Wnt signaling pathway Computational Biology Reproducibility of Results Gene expression profiling RNA Interference Algorithms Signal Transduction Genetic screen |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep00428 |
| Popis: | We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This technique can be applied to siRNA screen results retroactively to confirm positives and identify genes missed using conventional methods for on-target gene selection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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