Mitochondrial volume fraction and translation duration impact mitochondrial mRNA localization and protein synthesis
| Autor: | Evelina Tutucci, Fan Xu, Susanne M. Rafelski, Joonhyuk Choi, Ximena G. Arceo, Brian M. Zid, Raghav Chanchani, Robert H. Singer, Jingwen Yu, Yang S. Chen, Matheus P. Viana, Tatsuhisa Tsuboi |
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| Přispěvatelé: | Systems Bioinformatics, AIMMS |
| Rok vydání: | 2020 |
| Předmět: |
protein synthesis
RNA Mitochondrial Messenger Cell S. cerevisiae Mitochondrion Ribosome cell biology Gene expression Protein biosynthesis Biology (General) 0303 health sciences Chemistry General Neuroscience 030302 biochemistry & molecular biology Translation (biology) General Medicine Chromosomes and Gene Expression Mitochondrial Cell biology mitochondria Fungal medicine.anatomical_structure Medicine mRNA localization Research Article chromosomes QH301-705.5 1.1 Normal biological development and functioning Science Saccharomyces cerevisiae General Biochemistry Genetics and Molecular Biology Fungal Proteins Mitochondrial Proteins 03 medical and health sciences Underpinning research Genetics medicine RNA Messenger 030304 developmental biology Messenger RNA General Immunology and Microbiology RNA Fungal Cell Biology Cytoplasm Protein Biosynthesis gene expression RNA Generic health relevance Mitochondrial Size Biochemistry and Cell Biology |
| Zdroj: | eLife, Vol 9 (2020) Tsuboi, T, Viana, M P, Xu, F, Yu, J, Chanchani, R, Arceo, X G, Tutucci, E, Choi, J, Chen, Y S, Singer, R H, Rafelski, S M & Zid, B M 2020, ' Mitochondrial volume fraction and translation duration impact mitochondrial mRNA localization and protein synthesis ', eLife, vol. 9, e57814, pp. 1-24 . https://doi.org/10.7554/eLife.57814, https://doi.org/10.7554/ELIFE.57814 eLife, 9:e57814, 1-24. eLife Sciences Publications eLife |
| ISSN: | 2050-084X |
| Popis: | Mitochondria are dynamic organelles that must precisely control their protein composition according to cellular energy demand. Although nuclear-encoded mRNAs can be localized to the mitochondrial surface, the importance of this localization is unclear. As yeast switch to respiratory metabolism, there is an increase in the fraction of the cytoplasm that is mitochondrial. Our data point to this change in mitochondrial volume fraction increasing the localization of certain nuclear-encoded mRNAs to the surface of the mitochondria. We show that mitochondrial mRNA localization is necessary and sufficient to increase protein production to levels required during respiratory growth. Furthermore, we find that ribosome stalling impacts mRNA sensitivity to mitochondrial volume fraction and counterintuitively leads to enhanced protein synthesis by increasing mRNA localization to mitochondria. This points to a mechanism by which cells are able to use translation elongation and the geometric constraints of the cell to fine-tune organelle-specific gene expression through mRNA localization. |
| Databáze: | OpenAIRE |
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