New model substrates for enzymes hydrolysing polyethyleneterephthalate and polyamide fibres
| Autor: | Sonja Heumann, Anita Eberl, Georg Gübitz, Stefan Liebminger, Herbert Pobeheim, Eva Almansa, Artur Cavaco-Paulo, Gudrun Fischer-Colbrie |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2006 |
| Předmět: |
0106 biological sciences
Cutinase Biophysics 01 natural sciences Biochemistry Esterase Nocardia Amidohydrolases Substrate Specificity Amidase 03 medical and health sciences Hydrolysis Fusarium 010608 biotechnology Actinomycetales Organic chemistry Beauveria Lipase 030304 developmental biology chemistry.chemical_classification 0303 health sciences Science & Technology biology Polyethylene Terephthalates Chemistry Polyethyleneterphthalate Model substrate Esterases Substrate (chemistry) Enzyme assay Enzymes Nylons Aspergillus Enzyme Models Chemical Polyamide biology.protein Biotechnology Peptide Hydrolases |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 0165-022X |
| DOI: | 10.1016/j.jbbm.2006.02.005 |
| Popis: | Recently the potential of enzymes for surface hydrophilisation and/or functionalisation of polyethyleneterephthalate (PET) and polyamide (PA) has been discovered. However, there was no correlation between enzyme class/activity (e.g. esterase, lipase, cutinase) and surface hydrolysis of these polymers and consequently no simple assay to estimate this capability. Enzymes active on the model substrates bis (benzoyloxyethyl) terephthalate and adipic acid bishexyl-amide, were also capable of increasing the hydrophilicity of PET and PA. When dosed at the identical activity on 4-nitrophenyl butyrate, only enzymes from Thermobifida fusca, Aspergillus sp., Beauveria sp. and commercial enzymes (TEXAZYME PES sp5 and Lipase PS) increased the hydrophilicity of PET fibres while other esterases and lipases did not show any effect. Activity on PET correlated with the activity on the model substrate. Hydrophilicity of fibres was greatly improved based on increases in rising height of up to 4.3 cm and the relative decrease of water absorption time between control and sample of the water was up to 76%. Similarly, enzymes increasing the hydrophilicity of PA fibres such as from Nocardia sp., Beauveria sp. and F. solani hydrolysed the model substrate; however, there was no common enzyme activity (e.g. protease, esterase, amidase) which could be attributed to all these enzymes. |
| Databáze: | OpenAIRE |
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