Connective tissue growth factor gene expression alters tumor progression in esophageal cancer
| Autor: | David R. Brigstock, Alexander Koliopanos, Arthur Zimmermann, Helmut Friess, Markus W. Büchler, When-Hao Tang, Fabio F. di Mola, Darius Kubulus |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male medicine.medical_specialty Esophageal Neoplasms Blotting Western Gene Expression Adenocarcinoma Immediate-Early Proteins Fibrosis Transforming Growth Factor beta Internal medicine medicine Humans Northern blot RNA Messenger Growth Substances Aged Aged 80 and over Tumor microenvironment business.industry Connective Tissue Growth Factor Cancer Middle Aged medicine.disease Blotting Northern Immunohistochemistry Survival Analysis Extracellular Matrix CTGF Endocrinology Epidermoid carcinoma Tumor progression Cancer cell Cancer research Carcinoma Squamous Cell Disease Progression Intercellular Signaling Peptides and Proteins Surgery Female business Carrier Proteins Receptors Transforming Growth Factor beta |
| Zdroj: | World journal of surgery. 26(4) |
| ISSN: | 0364-2313 |
| Popis: | The ability of cancer cells to initiate specific fibroblast reactions may subsequently determine tumor evolution. In the present study we examined the coordinated expression of transforming growth factor-beta-1 (TGF-beta1), its signaling receptors, and its downstream mediator-connective tissue growth factor (CTGF)--and their impact on tumor progression and fibrogenesis in esophageal carcinomas. Messenger ribonucleic acid (mRNA) expression of TGF-beta1, CTGF, TGF-beta receptor subtype I ALK5 (TbetaR-IALK5), and TGF-beta receptor type II (TbetaR-II) was studied by Northern blot analysis in esophageal cancer and the normal esophagus. By means of immunohistochemistry and Western blot analysis, the respective proteins were localized in the tissue samples and the protein content was quantitated. Northern blot analysis revealed 3-fold and 4-fold increases (p0.05) in TGF-beta1 and CTGF mRNA levels, respectively, in esophageal cancer in comparison with normal controls, whereas TbetaR-I mRNA levels were significantly decreased and TbetaR-II mRNA levels were unchanged in the cancer samples. Immunostaining revealed results similar to those seen on the RNA level. TGF-beta1 and CTGF immunoreactivity were increased, TbetaR-II was unchanged, and TbetaR-IALK5 immunoreactivity was decreased. CTGF immunoreactivity was mainly present in the stroma surrounding the cancer cells but was also present in the cancer cells. The degree of fibrosis was different in squamous and adenocarcinomas and was significantly related to CTGF mRNA expression levels. The presence of CTGF in squamous cell carcinomas was associated with longer survival, whereas in adenocarcinomas it influenced survival negatively. The findings indicate that TGF-beta signaling is disturbed in esophageal cancer. CTGF, a downstream effector of TGF-beta action, differentially influences the composition of tumor microenvironment and distinct cell-matrix interactions in the two histological types of esophageal carcinoma, resulting in differences in tumor progression and patient survival. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink