Molecular characterization and phylogenetic analysis of Fasciola gigantica from western Java, Indonesia
| Autor: | Sodirun, Putut Eko Wibowo, Azirwan Guswanto, Uday Kumar Mohanta, Puttik Allamanda, Yoshifumi Nishikawa, Kei Hayashi, Madoka Ichikawa-Seki |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
China Buffaloes Fasciola gigantica Population Zoology Southeast asian Haplogroup 03 medical and health sciences Animals education Phylogeny DNA Polymerase III education.field_of_study Base Sequence biology Fasciola Phylogenetic tree Ecology Haplotype NADH Dehydrogenase Ruminants Sequence Analysis DNA DNA Helminth 030108 mycology & parasitology Thailand biology.organism_classification Infectious Diseases Vietnam Indonesia Parasitology Bubalus Phosphoenolpyruvate Carboxykinase (ATP) |
| Zdroj: | Parasitology International. 65:424-427 |
| ISSN: | 1383-5769 |
| DOI: | 10.1016/j.parint.2016.06.004 |
| Popis: | Fasciola gigantica and aspermic (hybrid) Fasciola flukes are thought to be distributed in Southeast Asian countries. The objectives of this study were to investigate the distribution of these flukes from unidentified ruminants in western Java, Indonesia, and to determine their distribution history into the area. Sixty Fasciola flukes from western Java were identified as F. gigantica based on the nucleotide sequences of the nuclear phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold) genes. The flukes were then analyzed phylogenetically based on the nucleotide sequence of the mitochondrial NADH dehydrogenase subunit 1 (nad1) gene, together with Fasciola flukes from other Asian countries. All but one F. gigantica fluke were classified in F. gigantica haplogroup C, which mainly contains nad1 haplotypes detected in flukes from Thailand, Vietnam, and China. A population genetic analysis suggested that haplogroup C spread from Thailand to the neighboring countries including Indonesia together with domestic ruminants, such as the swamp buffalo, Bubalus bubalis. The swamp buffalo is one of the important definitive hosts of Fasciola flukes in Indonesia, and is considered to have been domesticated in the north of Thailand. The remaining one fluke displayed a novel nad1 haplotype that has never been detected in the reference countries. Therefore, the origin of the fluke could not be established. No hybrid Fasciola flukes were detected in this study, in contrast to neighboring Asian countries. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect