The chromate resistance phenotype of some yeast mutants correlates with a lower level of Cr(V)-species generated in the extra-cellular medium
| Autor: | Mykhailo Gonchar, Galina Gayda, Yuriy Usatenko, Helena Ksheminska, Taras Honchar |
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| Rok vydání: | 2010 |
| Předmět: |
Pichia guilliermondii
Chromate conversion coating business.industry Mutant Electron Spin Resonance Spectroscopy Metals and Alloys Plant physiology Biology Phenotype Pichia General Biochemistry Genetics and Molecular Biology Yeast Culture Media Biotechnology Biomaterials Biochemistry Mutation Chromates CHROMATE RESISTANCE General Agricultural and Biological Sciences business Gene |
| Zdroj: | BioMetals. 23:633-642 |
| ISSN: | 1572-8773 0966-0844 |
| DOI: | 10.1007/s10534-010-9317-1 |
| Popis: | The paper describes the selection of chromate-resistant mutants of the yeast Pichia guilliermondii with a higher chromate-reducing activity and reports the EPR-study of Cr(V)-generation in the extra-cellular medium during the reduction of chromate by the yeast culture. It is shown that the reduction of chromate to Cr(III) species runs through the extra-cellular generation of Cr(V)-intermediate(s), thus supporting the assumption about the existence of an extra-cellular pathway of Cr(VI)-reduction. Furthermore, it is demonstrated that the chromate-resistance phenotype of tested mutants correlates with a lower stationary level of Cr(V)-species in the medium. It is thus suggested that isolated mutants can be used as sources of Cr(III)-biocomplexes due to their ability to effectively reduce chromate to Cr(III)-chelates with potential pharmacological applications. |
| Databáze: | OpenAIRE |
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