Serotonin-induced changes in L-leucine transport across rabbit jejunum
| Autor: | M.J. Rodriguez-Yoldi, M.-C. Rodríguez-Yoldi, M.T. Salvador, Ana I. Alcalde, R. Marco |
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| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Serotonin Brush border Biology General Biochemistry Genetics and Molecular Biology Intestinal absorption Diffusion Leucine Internal medicine Gallic Acid medicine Staurosporine Animals General Pharmacology Toxicology and Pharmaceutics Enzyme Inhibitors Protein kinase C Protein Kinase C chemistry.chemical_classification Sodium Biological Transport General Medicine Intestinal epithelium Trifluoperazine Amino acid Enzyme Activation Endocrinology Jejunum chemistry Intestinal Absorption Tetradecanoylphorbol Acetate Rabbits Intracellular medicine.drug |
| Zdroj: | Life sciences. 59(15) |
| ISSN: | 0024-3205 |
| Popis: | The aim of the present study has been to determine the effect of serotonin (5-HT) on the absorption of L-leucine across the rabbit jejunum. The results show that serotonin significantly diminishes the uptake and steady-state tissue accumulation and the mucosal to serosal flux of L-leucine. This effect does not change with previous intestinal exposure of the mucosa to the 5-HT. Serotonin does not seem to modify amino acid simple diffusion across the intestinal epithelium. The effect on the amino acid uptake is due to an inhibition of the Na + -dependent system of transport, mainly through a reduction of the apparent Vmax. Moreover, this hormone, added to the incubation solution, does not affect the L-leucine uptake across brush border membrane vesicles. In presence of trifluoroperazine, TMB-8 and staurosporine, the serotonin effect disappears. These results suggest an effect mediated by intracellular processes related to protein kinase C which inhibit the intestinal absorption of L-leucine. |
| Databáze: | OpenAIRE |
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