Harmonization of lateral fluid-percussion injury model production and post-injury monitoring in a preclinical multicenter biomarker discovery study on post-traumatic epileptogenesis
Autor: | Matthew R. Hudson, Noora Puhakka, Riikka Immonen, Emma L. Braine, Gregory Smith, Xavier Ekolle Ndode-Ekane, Richard J. Staba, César Emmanuel Santana-Gómez, Rhys D. Brady, Idrish Ali, Asla Pitkänen, Pedro Andrade, Pablo M. Casillas-Espinosa, Terence J. O'Brien, Sandy R. Shultz |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Male International Cooperation Neurodegenerative Epileptogenesis Rats Sprague-Dawley Epilepsy Traumatic brain injury 0302 clinical medicine Biomarker discovery Post-traumatic epilepsy Statistics Apnea Electroencephalography Magnetic Resonance Imaging Multicenter study Neurology Fluid percussion Anticonvulsants Female medicine.symptom medicine.medical_specialty Physical Injury - Accidents and Adverse Effects Clinical Sciences Traumatic Brain Injury (TBI) Post injury Statistics Nonparametric Article 03 medical and health sciences medicine Animals Humans Nonparametric Traumatic Head and Spine Injury Neurology & Neurosurgery Post-traumatic epileptogenesis Animal business.industry Neurosciences medicine.disease Epilepsy Post-Traumatic Brain Disorders Rats Disease Models Animal Good Health and Well Being 030104 developmental biology Brain Injuries Disease Models Emergency medicine Post-Traumatic Sprague-Dawley Neurology (clinical) business Biomarkers 030217 neurology & neurosurgery |
Zdroj: | Epilepsy research. 151 |
ISSN: | 1872-6844 |
Popis: | Multi-center preclinical studies can facilitate the discovery of biomarkers of antiepileptogenesis and thus facilitate the diagnosis and treatment development of patients at risk of developing post-traumatic epilepsy. However, these studies are often limited by the difficulty in harmonizing experimental protocols between laboratories. Here, we assess whether the production of traumatic brain injury (TBI) using the lateral fluid-percussion injury (FPI) in adult male Sprague-Dawley rats (12 weeks at the time of injury) was harmonized between three laboratories - located in the University of Eastern Finland (UEF), Monash University in Melbourne, Australia (Melbourne) and The University of California, Los Angeles, USA (UCLA). These laboratories are part of the international multicenter-based project, the Epilepsy Bioinformatics Study for Antiepileptogenesis Therapy (EpiBioS4Rx). Lateral FPI was induced in adult male Sprague-Dawley rats. The success of methodological harmonization was assessed by performing inter-site comparison of injury parameters including duration of anesthesia during surgery, impact pressure, post-impact transient apnea, post-impact seizure-like behavior, acute mortality ( Melbourne > UCLA (p |
Databáze: | OpenAIRE |
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