Harmonization of lateral fluid-percussion injury model production and post-injury monitoring in a preclinical multicenter biomarker discovery study on post-traumatic epileptogenesis

Autor: Matthew R. Hudson, Noora Puhakka, Riikka Immonen, Emma L. Braine, Gregory Smith, Xavier Ekolle Ndode-Ekane, Richard J. Staba, César Emmanuel Santana-Gómez, Rhys D. Brady, Idrish Ali, Asla Pitkänen, Pedro Andrade, Pablo M. Casillas-Espinosa, Terence J. O'Brien, Sandy R. Shultz
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
International Cooperation
Neurodegenerative
Epileptogenesis
Rats
Sprague-Dawley
Epilepsy
Traumatic brain injury
0302 clinical medicine
Biomarker discovery
Post-traumatic epilepsy
Statistics
Apnea
Electroencephalography
Magnetic Resonance Imaging
Multicenter study
Neurology
Fluid percussion
Anticonvulsants
Female
medicine.symptom
medicine.medical_specialty
Physical Injury - Accidents and Adverse Effects
Clinical Sciences
Traumatic Brain Injury (TBI)
Post injury
Statistics
Nonparametric
Article
03 medical and health sciences
medicine
Animals
Humans
Nonparametric
Traumatic Head and Spine Injury
Neurology & Neurosurgery
Post-traumatic epileptogenesis
Animal
business.industry
Neurosciences
medicine.disease
Epilepsy
Post-Traumatic
Brain Disorders
Rats
Disease Models
Animal
Good Health and Well Being
030104 developmental biology
Brain Injuries
Disease Models
Emergency medicine
Post-Traumatic
Sprague-Dawley
Neurology (clinical)
business
Biomarkers
030217 neurology & neurosurgery
Zdroj: Epilepsy research. 151
ISSN: 1872-6844
Popis: Multi-center preclinical studies can facilitate the discovery of biomarkers of antiepileptogenesis and thus facilitate the diagnosis and treatment development of patients at risk of developing post-traumatic epilepsy. However, these studies are often limited by the difficulty in harmonizing experimental protocols between laboratories. Here, we assess whether the production of traumatic brain injury (TBI) using the lateral fluid-percussion injury (FPI) in adult male Sprague-Dawley rats (12 weeks at the time of injury) was harmonized between three laboratories - located in the University of Eastern Finland (UEF), Monash University in Melbourne, Australia (Melbourne) and The University of California, Los Angeles, USA (UCLA). These laboratories are part of the international multicenter-based project, the Epilepsy Bioinformatics Study for Antiepileptogenesis Therapy (EpiBioS4Rx). Lateral FPI was induced in adult male Sprague-Dawley rats. The success of methodological harmonization was assessed by performing inter-site comparison of injury parameters including duration of anesthesia during surgery, impact pressure, post-impact transient apnea, post-impact seizure-like behavior, acute mortality ( Melbourne > UCLA (p
Databáze: OpenAIRE
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