Pseudohypophosphataemia as a Result of Bilirubin Interference
| Autor: | G O Duncanson, H G J Worth |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Molybdenum
030213 general clinical medicine Chromatography Bilirubin Clinical Biochemistry Analytical chemistry Jaundice 030209 endocrinology & metabolism General Medicine Serum phosphate Phosphate Interference (wave propagation) Sensitivity and Specificity Phosphate measurement Serum bilirubin Phosphates 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Spectrophotometry Humans Phosphoric Acids Quantitative analysis (chemistry) |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 27:253-257 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1177/000456329002700312 |
| Popis: | During the routine use of a discrete analyser it was noted that, when the serum bilirubin concentration was greater than 50 µmol/L, there was interference with serum phosphate determination measured by the formation of unreduced phosphomolybdate using a bichromatic system of measurement. The degree of interference was assessed by comparison with a reduced phosphomolybdate method (molybdenum blue). The interference cannot be removed by changing the secondary wavelength or by the use of a sample blank. It is proportional to the serum bilirubin concentration, but is not significant when this is less than 50 µmol/L. The monochromatic non-reduced phosphomolybdate method compares well with the reduced method. |
| Databáze: | OpenAIRE |
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