The relative merits of the tetO2 and tetO7 promoter systems for the functional analysis of heterologous genes in yeast and a compilation of essential yeast genes with tetO2 promoter substitutions
| Autor: | Stephen G. Oliver, Kuangyu Yen, Paul Gitsham, Nianshu Zhang, Zorana Vujovic, Jill A. Wishart, J. Ross Miller, Manda E. Gent, Michael J. Osborn |
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| Rok vydání: | 2006 |
| Předmět: |
Saccharomyces cerevisiae
Genes Fungal Molecular Sequence Data Mutagenesis (molecular biology technique) lac operon Bioengineering Applied Microbiology and Biotechnology Biochemistry Polymerase Chain Reaction Bacterial Proteins Genetics Humans DNA Fungal Promoter Regions Genetic Transcription factor Gene biology Base Sequence Fungal genetics biology.organism_classification Yeast Mutagenesis Insertional Lac Operon Carrier Proteins Functional genomics Sequence Alignment Biotechnology Interferon Regulatory Factor-1 |
| Zdroj: | Yeast (Chichester, England). 23(4) |
| ISSN: | 0749-503X |
| Popis: | We have generated a collection of yeast strains, each of which has an essential yeast gene under the control of the tetracycline-responsive, tetO, promoter. Screens using first-generation promoter-swap strains uncovered the non-specific responsiveness of the tetO7 promoter to a known human transcription factor (hIRF-1). Non-specific regulation was not observed with the tetO2 promoter. Reporter assays have been used to demonstrate this phenomenon. Subsequent efforts to generate a collection of tetracycline-regulatable strains have focused on the tetO2 promoter. These strains are available to the yeast community and can be used for functional genomics studies. |
| Databáze: | OpenAIRE |
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