Non-invasive in vivo determination of viable islet graft volume by 111In-exendin-3

Autor: Karolina M. Andralojc, Desiree Bos, Mijke Buitinga, Martin Gotthardt, Stefanie Willekens, Wael A. Eter, Lieke Joosten, Inge van der Kroon, Otto C. Boerman, Cathelijne Frielink, Maarten Brom
Rok vydání: 2017
Předmět:
0301 basic medicine
medicine.medical_specialty
endocrine system
Science
Urology
Islets of Langerhans Transplantation
030209 endocrinology & metabolism
Single-photon emission computed tomography
Article
03 medical and health sciences
Islets of Langerhans
Mice
0302 clinical medicine
In vivo
Insulin-Secreting Cells
Tumours of the digestive tract Radboud Institute for Molecular Life Sciences [Radboudumc 14]
medicine
Animals
Tomography
Emission-Computed
Single-Photon
Type 1 diabetes
geography
Multidisciplinary
geography.geographical_feature_category
medicine.diagnostic_test
business.industry
Other Research Radboud Institute for Health Sciences [Radboudumc 0]
Indium Radioisotopes
medicine.disease
Islet
Immunohistochemistry
Surgery
Molecular Imaging
Transplantation
030104 developmental biology
Medicine
Autoradiography
Pancreatic islet transplantation
Female
Molecular imaging
business
Peptides
Nanomedicine Radboud Institute for Molecular Life Sciences [Radboudumc 19]
Preclinical imaging
Zdroj: Scientific Reports
Scientific Reports, 7
Scientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
ISSN: 2045-2322
DOI: 10.1038/s41598-017-07815-3
Popis: Pancreatic islet transplantation is a promising therapy for patients with type 1 diabetes. However, the duration of long-term graft survival is limited due to inflammatory as well as non-inflammatory processes and routine clinical tests are not suitable to monitor islet survival. 111In-exendin-SPECT (single photon emission computed tomography) is a promising method to non-invasively image islets after transplantation and has the potential to help improve the clinical outcome. Whether 111In-exendin-SPECT allows detecting small differences in beta-cell mass (BCM) and measuring the actual volume of islets that were successfully engrafted has yet to be demonstrated. Here, we evaluated the performance of 111In-exendin-SPECT using an intramuscular islet transplantation model in C3H mice. In vivo imaging of animals transplanted with 50, 100, 200, 400 and 800 islets revealed an excellent linear correlation between SPECT quantification of 111In-exendin uptake and insulin-positive area of islet transplants, demonstrating that 111In-exendin-SPECT specifically and accurately measures BCM. The high sensitivity of the method allowed measuring small differences in graft volumes, including grafts that contained less than 50 islets. The presented method is reliable, convenient and holds great potential for non-invasive monitoring of BCM after islet transplantation in humans.
Databáze: OpenAIRE
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