Genomovar assignment of Pseudomonas stutzeri populations inhabiting produced oil reservoirs
| Autor: | Yi Shaojin, Liuqin Huang, Zhengliang Wang, Dujie Hou, Hailiang Dong, Ibrahim M. Banat, Yuehui She, Lujun Chai, Fan Zhang |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Microorganism Molecular Sequence Data Zoology Biology Microbiology DNA Ribosomal Phylogenetics RNA Ribosomal 16S oil reservoirs Genetic variation Cluster Analysis Oil and Gas Fields Oil field Phylogeny Original Research Pseudomonas stutzeri Genomovar Genetic Variation Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification Genetic structure 16S rRNA gene |
| Zdroj: | MicrobiologyOpen |
| ISSN: | 2045-8827 |
| Popis: | Oil reservoirs are specific habitats for the survival and growth of microorganisms in general. Pseudomonas stutzeri which is believed to be an exogenous organism inoculated into oil reservoirs during the process of oil production was detected frequently in samples from oil reservoirs. Very little is known, however, about the distribution and genetic structure of P. stutzeri in the special environment of oil reservoirs. In this study, we collected 59 P. stutzeri 16S rRNA gene sequences that were identified in 42 samples from 25 different oil reservoirs and we isolated 11 cultured strains from two representative oil reservoirs aiming to analyze the diversity and genomovar assignment of the species in oil reservoirs. High diversity of P. stutzeri was observed, which was exemplified in the detection of sequences assigned to four known genomovars 1, 2, 3, 20 and eight unknown genomic groups of P. stutzeri. The frequent detection and predominance of strains belonging to genomovar 1 in most of the oil reservoirs under study indicated an association of genomovars of P. stutzeri with the oil field environments. |
| Databáze: | OpenAIRE |
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