Gastroprotective activity of chloroform extract ofMuntingia calaburaandMelastoma malabathricumleaves
| Autor: | Siti Syariah Mamat, Zainul Amiruddin Zakaria, Siti Farah Md Tohid, Nurul Izzah Salleh, Farah Hidayah Kamisan, Nurliana Nasir, Norhafizah Mohtarrudin, Lay Kek Teh, Siti Selina Abdul Hamid, Amal Syahirah Nur Zainol, Azfar Hizami, Mohd Zaki Salleh, Adibah Sahmat, Nur Diyana Mahmood |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Antioxidant medicine.medical_treatment Pharmaceutical Science Pharmacology 030226 pharmacology & pharmacy Antioxidants Rats Sprague-Dawley Terpene 03 medical and health sciences 0302 clinical medicine Drug Discovery medicine Gastric mucosa Animals Omeprazole Melastoma malabathricum biology Traditional medicine Plant Extracts Chemistry General Medicine biology.organism_classification Rats Plant Leaves medicine.anatomical_structure Complementary and alternative medicine Phytochemical Gastric Mucosa Polyphenol 030220 oncology & carcinogenesis Melastomataceae Molecular Medicine Chloroform Muntingiaceae medicine.drug |
| Zdroj: | Pharmaceutical Biology. 54:812-826 |
| ISSN: | 1744-5116 1388-0209 |
| DOI: | 10.3109/13880209.2015.1085580 |
| Popis: | Muntingia calabura L. (family Muntingiaceae) and Melastoma malabathricum L. (family Melastomaceae) are traditionally used to treat gastric ulcer.The present study determines the mechanisms of gastroprotective activity of the chloroform extract of leaves obtained from both the plants using several in vitro and in vivo assays.Phytochemical screening, HPLC analysis, and antioxidant activity of the respective extract were carried out. Gastroprotective activity was determined using ethanol-induced gastric ulcer assay while the mechanisms of gastroprotection were determined using the pyloric ligation assay. The test solutions [8% Tween-80 (vehicle), 20 mg/kg omeprazole, and different doses of extracts (50, 250, or 500 mg/kg] were administered orally once daily for 7 consecutive days before the animals were subjected to ethanol induced gastric ulcers.The chloroform-extracted M. calabura (CEMC) contains tannins, polyphenolics, triterpenes, and steroids while the chloroform-extracted M. malabathricum (CEMM) contains only triterpenes and steroids. CEMC, but not CEMM, exerted remarkably strong antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH)- (86% versus 16%) and superoxide- (73% versus 36%) radical scavenging assays. Both extracts demonstrated significant (p 0.05) gastroprotection with the EC50 value recorded at 192.3 or 297.7 mg/kg, respectively. In the pylorus ligation assay, CEMC and CEMM significantly (p 0.05) reduced the total and free acidity and volume; while increased the pH of gastric juice as well as the gastric wall mucus content in comparison with the vehicle-treated group.CEMC and CEMM exert gastroprotective effects in animals with ethanol-induced gastric ulcers via antioxidant and anti-secretory effects. |
| Databáze: | OpenAIRE |
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