Role of Caveolin-1 for Blocking the Epithelial-Mesenchymal Transition in Proliferative Vitreoretinopathy
| Autor: | Seiichi Kato, Takeshi Senga, Yasuhito Funahashi, Shiang-Jyi Hwang, Hiroki Kaneko, Hideyuki Shimizu, Akiko Higuchi, Fuxiang Ye, Takeshi Iwase, Shu Kachi, Tetsu Asami, Keiko Kataoka, Yosuke Nagasaka, Hiroko Terasaki, Kei Takayama |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proliferative vitreoretinopathy Epithelial-Mesenchymal Transition Blotting Western Caveolin 1 Cell Count Retinal Pigment Epithelium Real-Time Polymerase Chain Reaction Pathogenesis 03 medical and health sciences Mice 0302 clinical medicine Downregulation and upregulation Cell Movement medicine Animals Humans Epithelial–mesenchymal transition Cells Cultured Mice Knockout Retinal pigment epithelium business.industry Reverse Transcriptase Polymerase Chain Reaction Vitreoretinopathy Proliferative Cell migration medicine.disease Immunohistochemistry eye diseases Mice Inbred C57BL Disease Models Animal 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation 030221 ophthalmology & optometry Cancer research RNA sense organs business |
| Zdroj: | Investigative ophthalmologyvisual science. 58(1) |
| ISSN: | 1552-5783 |
| Popis: | Purpose Proliferative vitreoretinopathy (PVR) is one of the most severe ocular diseases. Fibrotic changes in retinal cells are considered to be involved in the pathogenesis of PVR. Epithelial-mesenchymal transition (EMT) of RPE cells is one of the main concepts in the pathogenesis of fibrovascular membranes (FVMs) in PVR. In this study, we examined the expression of Caveolin-1 in human FVMs from patients with PVR. We also examined the role of Caveolin-1 in the pathogenesis of PVR. Methods Western blotting, real-time PCR, and immunohistochemistry were performed with human FVMs and mouse eyes with PVR. Cell migration assays were performed to evaluate the involvement of Caveolin-1 in EMT using primary human and mouse RPE cells. Results Caveolin-1 was expressed in human FVMs and upregulated in the mouse eyes with PVR. The alpha-smooth muscle actin (αSMA) expression and migration ability were increased in RPE cells with knockout or knockdown of Caveolin-1, whereas zonula occludens-1 (ZO-1) immunohistochemistry showed reduced morphology and expression of ZO-1. In addition, migration assays showed that Caveolin-1 reduction increased RPE cell migration abilities. Conclusions These results indicated that Caveolin-1 in RPE cells prevents PVR by blocking EMT. |
| Databáze: | OpenAIRE |
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