The identification of tuberculosis biomarkers in human urine samples
| Autor: | Zandile Mlamla, Jonathan M. Blackburn, Jonathan Peter, Putuma P. Gqamana, Teri Roberts, Brandy L. Young, Grant Theron, Salome Smit, Ureshnie Govender, Keertan Dheda |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Proteomics Pulmonary and Respiratory Medicine Tuberculosis Point-of-Care Systems Urinary system Urine Disease Urinalysis Mass Spectrometry Mycobacterium tuberculosis South Africa Tuberculosis diagnosis Latent Tuberculosis medicine Humans biology business.industry Reproducibility of Results Middle Aged Prognosis medicine.disease biology.organism_classification Immunology Proteome Female business Biomarkers Chromatography Liquid |
| Zdroj: | European Respiratory Journal. 43:1719-1729 |
| ISSN: | 1399-3003 0903-1936 |
| Popis: | We aimed to determine whether shotgun proteomic approaches could be used to identify tuberculosis (TB)-specific biomarkers in the urine of well-characterised patients with active TB versus no TB. Patients with suspected TB (n=63) were classified as: definite TB (Mycobacterium tuberculosis positive culture, n=21); presumed latent-TB infection (LTBI) (M. tuberculosis negative culture, no radiological features of active TB, a positive QuantiFERON-TB Gold In-Tube (QFT-IT) test and a positive T-SPOT.TB test, n=24); and presumed non-TB/non-LTBI (M. tuberculosis negative culture, no radiological features of active TB, a negative QFT-IT test and a negative T-SPOT.TB test, n=18). Urine proteins, in the range of 3-50 kDa, were collected, separated by a one-dimensional SDS-PAGE gel and digested using trypsin, after which high-performance liquid chromatography-tandem mass spectrometry was used to identify the urinary proteome. 10 mycobacterial proteins were observed exclusively in the urine of definite TB patients, while six mycobacterial proteins were found exclusively in the urine of presumed LTBI patients. In addition, a gene ontology enrichment analysis identified a panel of 20 human proteins that were significant discriminators (p |
| Databáze: | OpenAIRE |
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