Reduced expressions of Foxp1 and Rassf1a genes in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine in rats
| Autor: | Ayumi Kato, Kyoko Shimizu, Madoka Hanaoka, Yozo Shimoichi, Toshifumi Tsujiuchi, Mayumi Shigemura, Daisei Hinotsume, Kanya Honoki |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Lung Neoplasms Nitrosamines Tumor suppressor gene Down-Regulation Biology Adenocarcinoma medicine.disease_cause chemistry.chemical_compound Carcinoma Non-Small-Cell Lung medicine Animals RNA Messenger Rats Wistar Gene Lung Tumor Suppressor Proteins Forkhead Transcription Factors FOXP1 Molecular biology Reverse transcriptase Rats Gene Expression Regulation Neoplastic Repressor Proteins medicine.anatomical_structure Cell Transformation Neoplastic Oncology chemistry Nitrosamine Amine gas treating Carcinogenesis |
| Zdroj: | Cancer letters. 236(2) |
| ISSN: | 0304-3835 |
| Popis: | To clarify the involvement of the Foxp1 and Rassf1a genes in lung carcinogenesis, we investigated their expressions in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP) in rats. Six week old male Wistar rats were given 2000 ppm BHP in their drinking water for 12 weeks and maintained without further treatment until they were sacrificed at 25 weeks. A total of 10 lung adenocarcinomas were obtained, along with the total RNA from each for assessment of expression by reverse transcription (RT)-polymerase chain reaction (PCR). The reduced expressions of the Foxp1 and Rassf1a genes were observed in some of the lung adenocarcinomas. These analyses were also confirmed by real-time quantitative RT-PCR. These results suggest that reduced expressions of Foxp1 and Rassf1a genes may play a role in the development of lung adenocarcinomas induced by BHP in rats. |
| Databáze: | OpenAIRE |
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