Dietary Vitamin D Supplementation Is Ineffective in Preventing Murine Cow's Milk Allergy, Irrespective of the Presence of Nondigestible Oligosaccharides
| Autor: | Kerperien, JoAnn, Veening-Griffioen, Désirée, Oja, Anna, Wehkamp, Tjalling, Jeurink, Prescilla V, Garssen, Johan, Knippels, Leon M J, Willemsen, Linette E M, Pharmacology, Pharmaceutics, Afd Pharmacology, Afd Pharmaceutics |
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| Přispěvatelé: | Pharmacology, Pharmaceutics, Afd Pharmacology, Afd Pharmaceutics, Graduate School, AII - Inflammatory diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Allergy medicine.medical_treatment Immunology T cells Oligosaccharides Milk allergy medicine.disease_cause T-Lymphocytes Regulatory Dendritic cells Cow's milk allergy Mice Immune system Allergen Internal medicine medicine Vitamin D and neurology Nondigestible oligosaccharides Animals Humans Immunology and Allergy Mesenteric lymph nodes Vitamin D Sensitization Skin Mice Inbred C3H business.industry General Medicine Regulatory T cells Allergens Immunoglobulin E medicine.disease Regulatory Diet Disease Models Animal Milk Endocrinology medicine.anatomical_structure Cow’s milk allergy Dietary Supplements Experimental Allergy − Research Article Cattle Female Milk Hypersensitivity business Adjuvant |
| Zdroj: | International archives of allergy and immunology, 181(12), 908-918. S. Karger AG International Archives of Allergy and Immunology, 181(12), 908. S. Karger AG Int Arch Allergy Immunol |
| ISSN: | 1018-2438 |
| DOI: | 10.1159/000509750 |
| Popis: | Introduction: Cow’s milk allergy (CMA) is one of the most common food allergies especially early in life. A mixture of nondigestible short-chain galacto-oligosaccharides, long-chain fructo-oligosaccharides, and pectin-derived acidic-oligosaccharides (GFA) may reduce allergy development and allergic symptoms in murine CMA. Recently, vitamin D (VitD) has been suggested to have beneficial effects in reducing allergy as well. Objective: In this study, the immune modulatory effect on allergy prevention using the combination of GFA and VitD was investigated. Methods: Female C3H/HeOuJ mice were fed a control or GFA-containing diet with depleted, standard (1,000 IU/kg), or supplemented (5,000 IU/kg) VitD content for 2 weeks before and during whey sensitization (n = 10–15). Mice were sensitized 5 times intragastrically with PBS as a control, whey as cow’s milk allergen, and/or cholera toxin as adjuvant on a weekly interval. One week after the last sensitization, mice were intradermally challenged in both ear pinnae and orally with whey, subsequently the acute allergic skin response and shock symptoms were measured. After 18 h, terminal blood samples, mesenteric lymph nodes, and spleens were collected. Whey-specific immunoglobulin (Ig) E and IgG1 levels were measured by means of ELISA. T cell subsets and dendritic cells (DCs) were studied using flow cytometry. Results: Additional VitD supplementation did not lower the allergic symptoms compared to the standard VitD diet. CMA mice fed the GFA diet supplemented with VitD (GFA VitD+) significantly decreased the acute allergic skin response of whey sensitized mice when compared to the CMA mice fed VitD (VitD+) group (p < 0.05). The effect of GFA was not improved by extra VitD supplementation even though the CMA mice fed the GFA VitD+ diet had a significantly increased percentage of CD103+ DCs compared to the VitD+ group (p < 0.05). The VitD-deprived mice showed a high percentage of severe shock and many reached the humane endpoint; therefore, these groups were not further analyzed. Conclusions: High-dose VitD supplementation in mice does not protect against CMA development in the presence or absence of GFA. |
| Databáze: | OpenAIRE |
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