Stepwise cytoskeletal polarization as a series of checkpoints in innate but not adaptive cytolytic killing
| Autor: | Christoph Wülfing, John D. Schatzle, Bozidar Purtic, Jennifer Klem |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Time Factors
Green Fluorescent Proteins Antineoplastic Agents Mice SCID Biology Models Biological Peptides Cyclic Mice Tubulin Depsipeptides Cytotoxic T cell Animals Cytoskeleton Cytolytic granule Multidisciplinary Lymphokine-activated killer cell Effector Nocodazole Biological Sciences Acquired immune system Actins Cell biology Killer Cells Natural CTL Luminescent Proteins Cell killing Lytic cycle T-Lymphocytes Cytotoxic |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 100(13) |
| ISSN: | 0027-8424 |
| Popis: | Cytolytic killing is a major effector mechanism in the elimination of virally infected and tumor cells. The innate cytolytic effectors, natural killer (NK) cells, and the adaptive effectors, cytotoxic T cells (CTL), despite differential immune recognition, both use the same lytic mechanism, cytolytic granule release. Using live cell video fluorescence microscopy in various primary cell models of NK cell and CTL killing, we show here that on tight target cell contact, a majority of the NK cells established cytoskeletal polarity required for effective lytic function slowly or incompletely. In contrast, CTLs established cytoskeletal polarity rapidly. In addition, NK cell killing was uniquely sensitive to minor interference with cytoskeletal dynamics. We propose that the stepwise NK cell cytoskeletal polarization constitutes a series of checkpoints in NK cell killing. In addition, the use of more deliberate progression to effector function to compensate for inferior immune recognition specificity provides a mechanistic explanation for how the same effector function can be used in the different functional contexts of the innate and adaptive immune response. |
| Databáze: | OpenAIRE |
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