Activation and Stabilization of Olive Recombinant 13-Hydroperoxide Lyase Using Selected Additives
| Autor: | Magali Mariani, Virginie Brunini-Bronzini de Caraffa, Jean Costa, Liliane Berti, Jean-Marie Desjobert, Alain Muselli, Claude Gambotti, Félix Tomi, Sophie Vincenti, Jacques Maury, S. Jacopini |
|---|---|
| Přispěvatelé: | Laboratoire de Biochimie et Biologie Moléculaire Végétales - UMR6134, Université Pascal Paoli (UPP)-Centre National de la Recherche Scientifique (CNRS), Sciences pour l'environnement (SPE), Centre National de la Recherche Scientifique (CNRS)-Université Pascal Paoli (UPP), Laboratoire de Chimie des Produits Naturels -UMR 6134 |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
[SDV]Life Sciences [q-bio] Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Hexanal law.invention chemistry.chemical_compound Reaction rate constant Cytochrome P-450 Enzyme System law [CHIM.ANAL]Chemical Sciences/Analytical chemistry 010608 biotechnology Olea Hydroperoxide lyase Enzyme Stability Glycerol Molecular Biology ComputingMilieux_MISCELLANEOUS Aldehyde-Lyases Plant Proteins Chromatography biology General Medicine Enzyme assay Recombinant Proteins Chloroplast Enzyme Activation chemistry Glycine [SDE]Environmental Sciences Recombinant DNA biology.protein 010606 plant biology & botany Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology Applied Biochemistry and Biotechnology, Humana Press, 2017, 182 (3), pp.1000-1013. ⟨10.1007/s12010-016-2377-0⟩ |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-016-2377-0⟩ |
| Popis: | The stabilization of olive recombinant hydroperoxide lyases (rHPLs) was investigated using selected chemical additives. Two rHPLs were studied: HPL full-length and HPL with its chloroplast transit peptide deleted (matured HPL). Both olive rHPLs are relatively stable at 4 °C, and enzyme activity can be preserved (about 100% of the rHPL activities are maintained) during 5 weeks of storage at −20 or at −80 °C in the presence of glycerol (10%, v/v). Among the additives used in this study, glycine (2.5% w/v), NaCl (0.5 M), and Na2SO4 (0.25 M) provided the highest activation of HPL full-length activity, while the best matured HPL activity was obtained with Na2SO4 (0.25 M) and NaCl (1 M). Although the inactivation rate constants (k) showed that these additives inactivate both rHPLs, their use is still relevant as they strongly increase HPL activity. Results of C6-aldehyde production assays also showed that glycine, NaCl, and Na2SO4 are appropriate additives and that NaCl appears to be the best additive, at least for hexanal production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink