Rapid and reliable MALDI-TOF mass spectrometry identification of Candida non-albicans isolates from bloodstream infections
| Autor: | Monica Cerullo, Giovanna Pulcrano, Maria Rosaria Catania, Fabio Rossano, Alessandra Tucci, Matilde Esposito, Antonio Vollaro, Dora Vita Iula |
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| Přispěvatelé: | Pulcrano, Giovanna, Iula, VITA DORA, Vollaro, A, Tucci, A, Cerullo, M, Esposito, M, Rossano, Fabio, Catania, MARIA ROSARIA |
| Rok vydání: | 2013 |
| Předmět: |
MALDI-TOF
Microbiology (medical) Biology Candida parapsilosis Mass spectrometry Microbiology Mycological Typing Techniques Bloodstream infection medicine Cluster Analysis Humans Blood culture Molecular Biology Phylogeny Candida medicine.diagnostic_test Candida glabrata Candidemia biology.organism_classification Yeast Candida non-albicans Non albicans candida Vitek II Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Bloodstream infections |
| Zdroj: | Journal of Microbiological Methods. 94:262-266 |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/j.mimet.2013.07.001 |
| Popis: | Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) fingerprinting has recently become an effective instrument for rapid microbiological diagnostics and in particular for identification of micro-organisms directly in a positive blood culture. The aim of the study was to evaluate a collection of 82 stored yeast isolates from bloodstream infection, by MALDI-TOF MS; 21 isolates were identified also directly from positive blood cultures and in the presence of other co-infecting micro-organisms. Of the 82 isolates grown on plates, 64 (76%) were correctly identified by the Vitek II system and 82 (100%) by MALDI-TOF MS; when the two methods gave different results, the isolate was identified by PCR. MALDI-TOF MS was unreliable in identifying two isolates (Candida glabrata and Candida parapsilosis) directly from blood culture; however, direct analysis from positive blood culture samples was fast and effective for the identification of yeast, which is of great importance for early and adequate treatment. |
| Databáze: | OpenAIRE |
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