The surface of articular cartilage contains a progenitor cell population
| Autor: | Joanna Bishop, Charles W. Archer, Paul Rooney, Sam Boyer, Zubeyde Bayram, Michael S. Wolfe, Brian Mark Thomson, Laura Haughton, Gary P. Dowthwaite, Ilyas M. Khan, Samantha Redman, Darrell J. R. Evans |
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| Rok vydání: | 2004 |
| Předmět: |
Cartilage
Articular Population Genetic Vectors Notch signaling pathway Receptors Cell Surface Chick Embryo Biology Chondrocyte Bone and Bones Tendons Chondrocytes Transduction Genetic Endopeptidases medicine Articular cartilage repair Cell Adhesion Animals Protease Inhibitors Progenitor cell Receptor Notch1 education Notch 1 Stem cell transplantation for articular cartilage repair education.field_of_study Cartilage Stem Cells Cell Biology Recombinant Proteins Cell biology Fibronectins medicine.anatomical_structure Immunology Cattle Amyloid Precursor Protein Secretases Cell Division Integrin alpha5beta1 Transcription Factors |
| Zdroj: | Journal of cell science. 117(Pt 6) |
| ISSN: | 0021-9533 |
| Popis: | It is becoming increasingly apparent that articular cartilage growth is achieved by apposition from the articular surface. For such a mechanism to occur, a population of stem/progenitor cells must reside within the articular cartilage to provide transit amplifying progeny for growth. Here, we report on the isolation of an articular cartilage progenitor cell from the surface zone of articular cartilage using differential adhesion to fibronectin. This population of cells exhibits high affinity for fibronectin, possesses a high colony-forming efficiency and expresses the cell fate selector gene Notch 1. Inhibition of Notch signalling abolishes colony forming ability whilst activated Notch rescues this inhibition. The progenitor population also exhibits phenotypic plasticity in its differentiation pathway in an embryonic chick tracking system, such that chondroprogenitors can engraft into a variety of connective tissue types including bone, tendon and perimysium. The identification of a chondrocyte subpopulation with progenitor-like characteristics will allow for advances in our understanding of both cartilage growth and maintenance as well as provide novel solutions to articular cartilage repair. |
| Databáze: | OpenAIRE |
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