Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli
| Autor: | Erick Denamur, Raymond Ruimy, Christine Amorin, Pierre Rouquet, Antoine Andremont, Bertrand Picard, David Skurnik |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Animals Wild Microbial Sensitivity Tests Biology medicine.disease_cause Integron Polymerase Chain Reaction Integrons law.invention Microbiology Feces Dogs Antibiotic resistance law Drug Resistance Bacterial Escherichia coli medicine Animals Humans Pharmacology (medical) Escherichia coli Infections Polymerase chain reaction Antibacterial agent Pharmacology Sequence Analysis DNA biology.organism_classification Antimicrobial Enterobacteriaceae Anti-Bacterial Agents Infectious Diseases Genes Bacterial Animals Domestic biology.protein |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 57:1215-1219 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkl122 |
| Popis: | Objectives: To determine the level of antimicrobial resistance and the occurrence of class 1, 2 and 3 integrons in faecal Escherichia coli from several animal populations variously exposed to human contact. Methods: A collection of 341 faecal E. coli isolates was constituted from several animal populations subject to various degrees of exposure to humans: 18 animals never exposed to humans (living in the Antarctic or Gabon), 71 wild animals living in a low human density area (mountainous region of the Pyrenees, France), 61 wild animals living in a higher human density area (Fontainebleau forest near Paris, France), and 128 extensively reared farm animals and 42 pet dogs, both living in the Pyrenees. Resistance to antimicrobial agents was determined by the method of disc diffusion and quantified using the resistance score of BE Murray, JJ Mathewson, HL DuPont, CD Ericsson and RR Reves (Antimicrobial Agents and Chemotherapy 1990; 34: 515–18). Integrons were characterized by triplex realtime PCR and sequencing. The absence of epidemiologic clones was confirmed by PCR-based methods. Results:Agradientofresistancerangingfromabsencetohighprevalence(resistancescoreof18.7%)anda gradualincreaseintheprevalenceofclass1integrons(from0%to16%),bothcorrelatedwiththeincreasein exposure to humans, were observed. In wild animals with little contact with humans, resistance, when present, was not mediated by integrons. Conclusions: Our findings firmly establish that thecurrent prevalence of antimicrobial resistance foundin animal faecal bacteria, as well as the prevalence of integrons, is clearly anthropogenic. The presence of integrons may constitute an adaptive process to environments whose antimicrobial pressure exceeds a certain threshold. |
| Databáze: | OpenAIRE |
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