Light scattering measurements supporting helical structures for chromatin in solution

Autor:	Rosalind I. Cotter, Ailsa M. Campbell, J. F. Pardon
Rok vydání:	1978
Předmět:	Cell Nucleus Erythrocytes Light Protein Conformation Solenoid (DNA) Biology Gyration Molecular physics Chromatin Light scattering Random coil Protein structure Biochemistry Ionic strength Helix Genetics Animals Nucleic Acid Conformation Scattering Radiation Nucleosome Chickens
Zdroj:	Nucleic Acids Research. 5:1571-1580
ISSN:	1362-4962 0305-1048
DOI:	10.1093/nar/5.5.1571
Popis:	Laser light scattering measurements have been made on a series of polynucleosomes containing from 50 to 150 nucleosomes. Radii of gyration have been determined as a function of polynucleosome length for different ionic strength solutions. The results suggest that at low ionic strength the chromatin adopts a loosely helical structure rather than a random coil. The helix becomes more regular on increasing the ionic strength, the dimension resembling those proposed by Finch and Klug for their solenoid model.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04902efbaad90b6ab656b2f549d9adf5 https://doi.org/10.1093/nar/5.5.1571 Zobrazit plný text záznamu