T cell receptor δ repertoire in inflamed and noninflamed colon of patients with IBD analyzed by CDR3 spectratyping
| Autor: | Wolfgang F. Caspary, Andreas Hennemann, Wolfgang Holtmeier, Rainer Duchmann, Ekkehard May |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cellular immunity
Colon Physiology T-Lymphocytes Molecular Sequence Data Gene Expression CDR3 Spectratyping Inflammatory bowel disease Diverticulitis Colonic Crohn Disease Intestinal mucosa Physiology (medical) medicine Humans Amino Acid Sequence RNA Messenger Intestinal Mucosa Crohn's disease Base Sequence Hepatology business.industry T-cell receptor Gastroenterology Receptors Antigen T-Cell gamma-delta T lymphocyte medicine.disease Complementarity Determining Regions Ulcerative colitis Immunology Colitis Ulcerative business |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 282:G1024-G1034 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.00224.2001 |
| Popis: | Gamma/delta T cells might play an important role in autoimmune conditions like inflammatory bowel disease (IBD). In the present study, we characterized the T cell receptor (TCR)-delta repertoire by complementarity determining region 3 (CDR3) spectratyping in the inflamed and noninflamed mucosa and in the peripheral blood of subjects with Crohn's disease and ulcerative colitis. In contrast to previously published data about alpha/beta T cells, we rarely found oligoclonal expansions of gamma/delta T cells specific only for the inflamed mucosa. The same dominant gamma/delta T cell expansions were also present in the noninflamed colon. Furthermore, the peripheral gamma/delta TCR repertoire was oligoclonal but clearly distinct from that in the inflamed intestine. Thus our results do not support a role for antigen-specific gamma/delta T cells in IBD, and dominant gamma/delta T cells of the peripheral blood are not likely to be derived from the inflamed gut. However, in several patients, the TCR-delta-repertoire was highly diversified, whereas in others we observed a loss of dominant gamma/delta T cell clones when inflamed and noninflamed mucosa were compared. In conclusion, those changes indicate that gamma/delta T cells might play an important role in a subset of patients with IBD. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|