Residual impact of 17α-methyltestosterone and histopathological changes in sex- reversed Nile tilapia (Oreochromis niloticus)
| Autor: | Muhammad Agus Suprayudi, Akhmad Taufiq Mukti, Epy Muhammad Luqman, Mirni Lamid, Dewi Nurmalita Suseno |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
food.ingredient Dose lcsh:Medicine Plant Science 03 medical and health sciences Nile tilapia 0302 clinical medicine food Animal science medicine Methyltestosterone 030219 obstetrics & reproductive medicine General Veterinary biology Aquaculture of tilapia lcsh:R 0402 animal and dairy science Obstetrics and Gynecology Tilapia 04 agricultural and veterinary sciences Sex reversal biology.organism_classification 040201 dairy & animal science Oreochromis Reproductive Medicine 17α-methyltestosterone residue organ histopathology tilapia sex reversal method Animal Science and Zoology Histopathology medicine.drug |
| Zdroj: | Asian Pacific Journal of Reproduction, Vol 9, Iss 1, Pp 37-43 (2020) |
| ISSN: | 2305-0500 |
| DOI: | 10.4103/2305-0500.275527 |
| Popis: | Objective: To examine sex reversal both by oral and by immersion using 17α-methyltestosterone on the methyltestosterone residual concentration and the organ histopathology of tilapia fish. Methods: This study used oral and immersion treatment methods for sex reversal of tilapia fish and used normal fish as the control and each treatment was repeated 4 times. 17α-methyltestosterone at dosages of 60 mg/kg feed and 0.5 mg/L were used for oral and immersion methods, respectively. In the first step, tilapia fry were reared at 100 L aquaria, with a density of 1 fish/L for 2 months. In the next step, male tilapias were reared at happa (net cage) of (2×1×1) m3 size in the controlled pond, with a density of 30 fish/happa for 3 months. The methyltestosterone residual concentrations were analyzed by one-way analysis of variance and Duncan’s multiple range tests, while organ histopathology was analyzed by descriptive method. Results: Residual concentrations in the serum of methyltestosterone- treated fish were significantly lower than that in normal fish, especially in 4- and 5-month-old tilapias with averages of less than 5 μg/L, while in normal fish was more than 5 μg/L. In the flesh, methyltestosterone residual concentrations showed relatively no significant differences between the oral and immersion treatment groups and methyltestosterone-treated fish remained lower compared to normal fish, except in 5-month-old tilapia. Methyltestosterone-treated tilapia exhibited histopathological changes on gill, liver, kidneys, and intestine organs. Conclusions: Sex reversal either by oral or by immersion has methyltestosterone residual concentration, but does not exceed the limits (5 μg/L or 5 μg/kg) of synthetic steroid on the fish body, although methyltestosterone causes histopathological changes on gill, liver, kidneys, and intestine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|